Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Ecological Science, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Ecological Science, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
Biochem Biophys Res Commun. 2022 Jan 1;586:81-86. doi: 10.1016/j.bbrc.2021.11.072. Epub 2021 Nov 19.
1-Deoxy-D-sorbitol, the 1-deoxy analogue of D-sorbitol, has been detected in human urine as well as in natural herbs and spices. Although there are sporadic reports about 1-deoxy-D-sorbitol dehydrogenase, the complete catabolic pathway of 1-deoxy-D-sorbitol remains unsolved. Informed by the promiscuous activities of fructose-6-phosphate aldolase (FSA) which is involved in the sorbitol (glucitol) utilization (gut) operon and guided by the large scale bioinformatics analysis, we predicted and then experimentally verified the gut operon encoded by Bacillus licheniformis ATCC14580 is responsible for the catabolism of both D-sorbitol and 1-deoxy-D-sorbitol by in vitro activity assays of pathway enzymes, in vivo growth phenotypes, and transcriptomic studies. Moreover, the phylogenetic distribution analysis suggests that the D-sorbitol and 1-deoxy-D-sorbitol catabolic gene cluster is mostly conserved in members of Firmicutes phylum.
1-脱氧-D-山梨醇是 D-山梨醇的 1-脱氧类似物，已在人尿以及天然草药和香料中被检测到。尽管有关于 1-脱氧-D-山梨醇脱氢酶的零星报道，但 1-脱氧-D-山梨醇的完整分解代谢途径仍未解决。受参与山梨醇（甘露醇）利用（肠道）操纵子的果糖-6-磷酸醛缩酶（FSA）的混杂活性的启发，并通过大规模生物信息学分析进行指导，我们预测并通过体外途径酶活性测定、体内生长表型和转录组学研究实验验证了由地衣芽孢杆菌 ATCC14580 编码的肠道操纵子负责 D-山梨醇和 1-脱氧-D-山梨醇的分解代谢。此外，系统发育分布分析表明，D-山梨醇和 1-脱氧-D-山梨醇分解代谢基因簇在厚壁菌门的成员中大多保守。
