电离辐射对 p21 基因转录变体表达的影响。

Effect of Ionizing Radiation on the Transcript Variants Expression of p21 Gene.

机构信息

Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Department of Virology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.

出版信息

Asian Pac J Cancer Prev. 2021 Nov 1;22(11):3717-3722. doi: 10.31557/APJCP.2021.22.11.3717.

DOI:10.31557/APJCP.2021.22.11.3717

PMID:34837932

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9068170/

Abstract

PURPOSE

CDK1A is one of the most important genes that have different key roles in cell lines. This gene has several transcript variants. Investigating of expression of each one actually can be so important because any one of them may have a separate unknown role in cancer cells so can be used to increase therapeutic efficacy.

METHODS

A549, MDA-MB-231 and Hek-AD cell lines were used in this study. Firstly, three primers for variants of p21 gene were designed by Snapgene and BLAST software. Secondly, the variants expression was checked for each cell lines by RT-qPCR technique, separately. Then the variants that expressed in the cells were selected for more investigation. Finally 2 Gy irradiation was used to evaluate the effect of that on variants expression.

RESULTS

The results show that for all cell lines, primer num1 and 3 expressed before any stimuli. After irradiation, for MDA-MB-231 and A549, the expression of primer num3 was decreased, while for Hek-AD no change was observed. The primer num1 expression after the irradiation was different for the cells, V1 expression was decreased in A549 by fold of 0.03 while expression of this for MDA-MB-231 cells was not changed after 2Gy irradiation.

CONCLUSION

It is very necessary to pay attention to the function of each splice variant as well as the response to external stimuli. Understanding the role of each variant in a gene is critical and researchers can use that to improve radiotherapy as well.

摘要

目的

CDK1A 是细胞系中具有不同关键作用的最重要基因之一。该基因有几个转录变体。研究每个变体的表达实际上非常重要，因为它们中的任何一个在癌细胞中可能具有单独的未知作用，因此可以用于提高治疗效果。

方法

本研究使用了 A549、MDA-MB-231 和 Hek-AD 细胞系。首先，通过 Snapgene 和 BLAST 软件设计了用于 p21 基因变体的三个引物。其次，通过 RT-qPCR 技术分别检查每个细胞系中变体的表达。然后选择在细胞中表达的变体进行进一步研究。最后，使用 2Gy 照射来评估其对变体表达的影响。

结果

结果表明，对于所有细胞系，引物 num1 和 3 在任何刺激之前表达。照射后，对于 MDA-MB-231 和 A549，引物 num3 的表达减少，而对于 Hek-AD 则没有观察到变化。照射后引物 num1 的表达在细胞之间是不同的，A549 中的 V1 表达减少了 0.03 倍，而 MDA-MB-231 细胞的表达在 2Gy 照射后没有变化。