人鼻病毒血清型可诱导不同的免疫应答。
Human rhinovirus serotypes induces different immune responses.
机构信息
Department of Otorhinolaryngology - Head and Neck Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.
出版信息
Virol J. 2021 Nov 27;18(1):232. doi: 10.1186/s12985-021-01701-1.
BACKGROUND
Different species of human rhinovirus (HRV) can induce varied antiviral and inflammatory responses in human blood macrophages and lower airway epithelium. Although human nasal epithelial cells (HNECs) are a primary infection route of HRV, differences between major and minor groups of HRV in the upper airway epithelium have not been studied in detail. In this study, we investigated viral replications and immune responses of major and minor groups of HRV in the HNECs.
METHODS
Viral replication, immune responses of IFN-β, IFN-λ, proinflammatory cytokines, and viral receptors, and mRNA expression of transcription factors of HRV16 (major group) and HRV1B (minor group) in the HNECs were assessed.
RESULTS
Compared with HRV16, HRV1B replicated more actively without excessive cell death and produced higher IFN-β, IFN-λ1/3, CXCL10, IL-6, IL-8, and IL-18 levels. Furthermore, low-density lipoprotein receptor (LDLR), TLR3, MDA5, NF-κB, STAT1, and STAT2 mRNA levels increased in HRV1B-infected HNECs.
CONCLUSION
HRV1B induces a stronger antiviral and inflammatory response from cell entry to downstream signaling compared with HRV16.
背景
不同种的人类鼻病毒（HRV）可在人血巨噬细胞和下呼吸道上皮细胞中诱导不同的抗病毒和炎症反应。虽然人鼻腔上皮细胞（HNECs）是 HRV 的主要感染途径，但在上呼吸道上皮细胞中，HRV 的主要组和次要组之间的差异尚未得到详细研究。在本研究中，我们研究了 HRV 的主要组和次要组在 HNECs 中的病毒复制和免疫反应。
方法
评估了 HRV16（主要组）和 HRV1B（次要组）在 HNECs 中的病毒复制、IFN-β、IFN-λ、促炎细胞因子和病毒受体的免疫反应，以及转录因子的 mRNA 表达。
结果
与 HRV16 相比，HRV1B 的复制更为活跃，细胞死亡较少，产生更高水平的 IFN-β、IFN-λ1/3、CXCL10、IL-6、IL-8 和 IL-18。此外，HRV1B 感染的 HNECs 中 LDLR、TLR3、MDA5、NF-κB、STAT1 和 STAT2 的 mRNA 水平增加。
结论
与 HRV16 相比，HRV1B 从细胞进入到下游信号转导诱导更强的抗病毒和炎症反应。
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