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工业黑曲霉菌株O1中多种酶对淀粉糖化的协同作用。

Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification.

作者信息

Guo Wenzhu, Yang Jianhua, Huang Tianchen, Liu Dandan, Liu Qian, Li Jingen, Sun Wenliang, Wang Xingji, Zhu Leilei, Tian Chaoguang

机构信息

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, China.

出版信息

Biotechnol Biofuels. 2021 Nov 27;14(1):225. doi: 10.1186/s13068-021-02074-x.

DOI:10.1186/s13068-021-02074-x
PMID:34838099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8627030/
Abstract

BACKGROUND

Starch is one of the most important renewable polysaccharides in nature for production of bio-ethanol. The starch saccharification step facilitates the depolymerization of starch to yield glucose for biofuels production. The filamentous fungus Aspergillus niger (A. niger) is the most used microbial cell factory for production of the commercial glucoamylase. However, the role of each component in glucoamylases cocktail of A. niger O1 for starch saccharification remains unclear except glucoamylase.

RESULTS

In this study, we identified the key enzymes contributing to the starch saccharification process are glucoamylase, α-amylase and acid α-amylase out of 29 glycoside hydrolases from the 6-day fermentation products of A. niger O1. Through the synergistic study of the multienzymes for the starch saccharification in vitro, we found that increasing the amount of α-amylase by 5-10 times enhanced the efficiency of starch saccharification by 14.2-23.2%. Overexpression of acid α-amylase in strain O1 in vivo increased the total glucoamylase activity of O1 cultures by 15.0%.

CONCLUSIONS

Our study clarifies the synergistic effects among the components of glucoamylases cocktail, and provides an effective approach to optimize the profile of saccharifying enzymes of strain O1 for improving the total glucoamylase activity.

摘要

背景

淀粉是自然界中用于生产生物乙醇的最重要的可再生多糖之一。淀粉糖化步骤有助于淀粉解聚以产生用于生物燃料生产的葡萄糖。丝状真菌黑曲霉是生产商业糖化酶最常用的微生物细胞工厂。然而，除糖化酶外，黑曲霉O1糖化酶混合物中各组分在淀粉糖化中的作用仍不清楚。

结果

在本研究中，我们从黑曲霉O1 6天发酵产物的29种糖苷水解酶中鉴定出对淀粉糖化过程有贡献的关键酶为糖化酶、α-淀粉酶和酸性α-淀粉酶。通过对多酶体外淀粉糖化的协同研究，我们发现将α-淀粉酶的量增加5-10倍可使淀粉糖化效率提高14.2-23.2%。体内在菌株O1中过表达酸性α-淀粉酶使O1培养物的总糖化酶活性提高了15.0%。

结论

我们的研究阐明了糖化酶混合物各组分之间的协同作用，并提供了一种优化菌株O1糖化酶谱以提高总糖化酶活性的有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/2c913d184b3b/13068_2021_2074_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/1311085bc747/13068_2021_2074_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/79aa90f18cd0/13068_2021_2074_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/750f3b3b829d/13068_2021_2074_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/e49ecf921e54/13068_2021_2074_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/2c913d184b3b/13068_2021_2074_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/1311085bc747/13068_2021_2074_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/79aa90f18cd0/13068_2021_2074_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/750f3b3b829d/13068_2021_2074_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/e49ecf921e54/13068_2021_2074_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21d9/8627030/2c913d184b3b/13068_2021_2074_Fig5_HTML.jpg

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本文引用的文献

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Essays Biochem. 2021 Jul 26;65(2):213-224. doi: 10.1042/EBC20200139.
2
The breakdown of starch in leaves.叶片中淀粉的分解
New Phytol. 2004 Aug;163(2):247-261. doi: 10.1111/j.1469-8137.2004.01101.x.
3
Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products.从枣类副产品中分离出的黑曲霉菌株胞外酶产生的特性及优化
Development of Genetic Tools in Glucoamylase-Hyperproducing Industrial Strains.
糖化酶高产工业菌株中遗传工具的开发
Biology (Basel). 2022 Sep 24;11(10):1396. doi: 10.3390/biology11101396.
J Genet Eng Biotechnol. 2021 Mar 31;19(1):50. doi: 10.1186/s43141-021-00145-y.
4
A Comprehensive Review on Corn Starch-Based Nanomaterials: Properties, Simulations, and Applications.基于玉米淀粉的纳米材料综述：性质、模拟与应用
Polymers (Basel). 2020 Sep 22;12(9):2161. doi: 10.3390/polym12092161.
5
Amylose in starch: towards an understanding of biosynthesis, structure and function.淀粉中的直链淀粉：对其生物合成、结构和功能的理解。
New Phytol. 2020 Dec;228(5):1490-1504. doi: 10.1111/nph.16858. Epub 2020 Sep 9.
6
Solid-state fermentation increases secretome complexity in Aspergillus brasiliensis.固态发酵增加了巴西曲霉的胞外分泌物复杂性。
Fungal Biol. 2020 Aug;124(8):723-734. doi: 10.1016/j.funbio.2020.04.006. Epub 2020 May 7.
7
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8
Succession of physiological stages hallmarks the transcriptomic response of the fungus to lignocellulose.生理阶段的相继出现标志着真菌对木质纤维素的转录组反应。
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10
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Microb Cell Fact. 2020 Apr 3;19(1):81. doi: 10.1186/s12934-020-01340-1.