Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology, Ministry of Education, School of Chemistry, Beihang University, Beijing 100191,China.
Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology, Ministry of Education, School of Chemistry, Beihang University, Beijing 100191,China; Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191,China.
Colloids Surf B Biointerfaces. 2022 Feb;210:112221. doi: 10.1016/j.colsurfb.2021.112221. Epub 2021 Nov 18.
Inspired by the ordered porous nanostructure of bone, biomimetic functionalization porous biomaterial could be considered as promising substitutes for bone regeneration. To realize the relevant biomimetic porous structure, polyvinyl alcohol (PVA)-based biomimetic cuttlebone aerogel scaffold which simultaneously contained modified carbon nanotubes (MCNTs) and hydroxyapatite (HAP) was first prepared using a one-step rapid freeze-drying method. By adjusting the MCNTs contents, both the surface hydrophilicity and the mechanical properties of the scaffold could be improved concurrently. Besides, the PVA/MCNTs/HAP enhanced the adhesion, differentiation and gene expression of osteogenic markers performances of MC3T3-E1 cells. Furthermore, the aerogel scaffolds were implanted into the calvarial defect model of SD IGS Rat to evaluate osteogenic performance in vivo. The Micro-CT characterization and bone content theoretical analysis after 8 weeks together indicated that the PVA/MCNTs/HAP aerogel scaffolds could accelerate bone regeneration without the contribution of endogenous cytokines. The unique biomimetic porous structure, superior mechanical properties and excellent bone regeneration capacity of PVA/MCNTs/HAP aerogel scaffolds made them potential materials for bone regeneration.
受骨的有序多孔纳米结构的启发，仿生功能化多孔生物材料可以被认为是骨再生的有前途的替代品。为了实现相关的仿生多孔结构，首先使用一步快速冷冻干燥法制备了同时含有改性碳纳米管（MCNT）和羟基磷灰石（HAP）的基于聚乙烯醇（PVA）的仿生乌贼骨气凝胶支架。通过调整 MCNT 的含量，可以同时提高支架的表面亲水性和机械性能。此外，PVA/MCNTs/HAP 增强了成骨标志物 MC3T3-E1 细胞的黏附、分化和基因表达性能。此外，将气凝胶支架植入 SD IGS 大鼠的颅顶骨缺损模型中，以评估体内成骨性能。8 周后的 Micro-CT 特征和骨含量理论分析表明，PVA/MCNTs/HAP 气凝胶支架可以在没有内源性细胞因子贡献的情况下加速骨再生。PVA/MCNTs/HAP 气凝胶支架独特的仿生多孔结构、优异的机械性能和出色的骨再生能力使它们成为骨再生的潜在材料。
