Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, P. R. China.
College of Basic Medical Science, Hebei University, Baoding 071000, P. R. China.
ACS Appl Bio Mater. 2021 Nov 15;4(11):8049-8059. doi: 10.1021/acsabm.1c00967. Epub 2021 Oct 25.
Two-dimensional materials provide a secluded space for bone formation and preserve the growth of surrounding tissues, thus playing a crucial role in guided bone regeneration (GBR). Graphene oxide (GO) has been widely employed in GBR due to its good mechanical and hydrophilic properties. A single GO membrane, however, does not provide a friendly environment for osteogenic cell adhesion. With their adjustable mechanical properties and excellent biocompatibility, composite membranes can simulate the multicomponent structure of an extracellular matrix for cell adhesion. To obtain two-dimensional membranes with appropriate mechanical strength and sufficient biocompatibility, GO-based composite membranes simultaneously containing chitosan (CS) and hydroxyapatite (HAP) were first prepared using one-step vacuum filtration and a biomimetic mineralization method. CS and HAP improved the mechanical strength and surface hydrophilicity of the membranes. In addition, moderate addition of HAP enhanced the adhesion, differentiation, and mineralization of osteoblasts. The prepared composite membranes were then implanted into a calvarial defect model to evaluate their osteogenic induction effects in vivo. Microcomputed tomography observation and histological analysis indicate that GO/CS/HAP composite membranes can accelerate bone regeneration without the contribution of endogenous cytokines. GO/CS/HAP composite membranes with unique biomimetic porous structures, superior mechanical properties, and excellent bone regeneration capacity are potential materials for application in GBR.
二维材料为骨形成提供了一个封闭的空间，并保留了周围组织的生长，因此在引导骨再生（GBR）中起着至关重要的作用。氧化石墨烯（GO）因其良好的机械性能和亲水性而在 GBR 中得到广泛应用。然而，单层 GO 膜不能为成骨细胞的黏附提供友好的环境。复合膜具有可调节的机械性能和优异的生物相容性，可以模拟细胞黏附的细胞外基质的多组分结构。为了获得具有适当机械强度和足够生物相容性的二维膜，首先采用一步真空过滤和仿生矿化法制备了同时含有壳聚糖（CS）和羟基磷灰石（HAP）的基于 GO 的复合膜。CS 和 HAP 提高了膜的机械强度和表面亲水性。此外，适量添加 HAP 可增强成骨细胞的黏附、分化和矿化。然后将制备的复合膜植入颅骨缺损模型中，以评估其体内的成骨诱导作用。微计算机断层扫描观察和组织学分析表明，GO/CS/HAP 复合膜可在没有内源性细胞因子贡献的情况下加速骨再生。GO/CS/HAP 复合膜具有独特的仿生多孔结构、优异的机械性能和出色的骨再生能力，是 GBR 应用的潜在材料。
