Abdelhalim Abdelsattar O E, Meshcheriakov Anatolii A, Maistrenko Dmitrii N, Molchanov Oleg E, Ageev Sergei V, Ivanova Daria A, Iamalova Nailia R, Luttsev Mikhail D, Vasina Lubov V, Sharoyko Vladimir V, Semenov Konstantin N
Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Prospect, Saint Petersburg 198504, Russia; Environmental Research Department, National Center for Social and Criminological Research (NCSCR), 4 Agouza, Giza, 11561, Egypt.
Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Prospect, Saint Petersburg 198504, Russia; Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo Street, Saint Petersburg 197022, Russia.
Colloids Surf B Biointerfaces. 2022 Feb;210:112232. doi: 10.1016/j.colsurfb.2021.112232. Epub 2021 Nov 20.
The article is dedicated to the comprehensive biocompatibility investigation of synthesised graphene oxide (GO) enriched with oxygen-containing functional groups (⁓85%). GO was synthesised through a modified Hummers and Offeman's method and characterised using C NMR, Raman, and IR spectroscopy, XRD, HRTEM, along with size dimensions and ζ-potentials in aqueous dispersions. Biocompatibility study included tests on haemocompatibility (haemolysis, platelet aggregation, binding to human serum albumin and its esterase activity), antioxidant activity (2,2-diphenyl-1-picrylhydrazyl reaction, NO-radical uptake, Radachlorin photobleaching, photo-induced haemolysis), genotoxicity using DNA comet assay, as well as metabolic activity and proliferation of HEK293 cells.
本文致力于对富含含氧官能团（约85%）的合成氧化石墨烯（GO）进行全面的生物相容性研究。GO通过改良的Hummers和Offeman方法合成，并利用碳核磁共振（C NMR）、拉曼光谱和红外光谱（IR）、X射线衍射（XRD）、高分辨率透射电子显微镜（HRTEM）以及水分散体中的尺寸大小和ζ电位进行表征。生物相容性研究包括血液相容性测试（溶血、血小板聚集、与人血清白蛋白结合及其酯酶活性）、抗氧化活性（2,2-二苯基-1-苦基肼反应、一氧化氮自由基摄取、拉达氯林光漂白、光诱导溶血）、使用DNA彗星试验的遗传毒性以及HEK293细胞的代谢活性和增殖。
