School of Hydraulic and Environmental Engineering, Changsha University of Science & Technology, Changsha 410004, PR China.
Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, PR China.
Bioresour Technol. 2022 Mar;347:126364. doi: 10.1016/j.biortech.2021.126364. Epub 2021 Nov 25.
The rapid start-up and stable operation of the single-stage partial nitritation-anammox (PNA) process remains a challenge in practical applications. An integrated investigation of nitrogen removal performance, sludge characteristics, activity and abundance, and microbial dynamics was implemented for 360 days via an airlift internal circulation reactor. During long-term operation, the reactor realized a stable dissolved oxygen (DO) partition and cultivated granular sludge. The nitrogen removal rate increased from 0.15 kg-N/m/d to 1.24 kg-N/m/d, and a high nitrogen removal efficiency of 82.6% was obtained. A stable DO partition further accelerated the bioreaction rates and enhanced the activity of functional microbes. The activities of ammonia oxidation and anammox reached 1.21 g-N/g-VSS/d and 1.43 g-N/g-VSS/d, respectively. Sludge granulation efficiently enriched the abundances of Candidatus Brocadia (7.4%) and Nitrosomonas (5.2%). These results demonstrated that efficient DO partition and stable culture of granular sludge could enhance the synergy of functional microbes for autotrophic nitrogen removal.
单级部分亚硝化-厌氧氨氧化（PNA）工艺的快速启动和稳定运行仍然是实际应用中的一个挑战。通过气升式内循环反应器，对氮去除性能、污泥特性、活性和丰度以及微生物动态进行了 360 天的综合研究。在长期运行过程中，反应器实现了稳定的溶解氧（DO）分配和颗粒污泥的培养。氮去除率从 0.15kg-N/m/d 增加到 1.24kg-N/m/d，获得了 82.6%的高氮去除效率。稳定的 DO 分配进一步加速了生物反应速率，并增强了功能微生物的活性。氨氧化和厌氧氨氧化的活性分别达到 1.21g-N/g-VSS/d 和 1.43g-N/g-VSS/d。污泥颗粒化有效地富集了Candidatus Brocadia（7.4%）和Nitrosomonas（5.2%）的丰度。这些结果表明，高效的 DO 分配和颗粒污泥的稳定培养可以增强功能微生物自养脱氮的协同作用。
