提取物对……的抗菌效果筛选。
Screening of antimicrobial effects of extract against .
作者信息
Sirimongkolvorakul Sunisa, Jasancheun Anusorn
机构信息
Department of Pre-Clinical Veterinary Science, Faculty of Veterinary Medicine, Mahanakorn University of Technology, 140 Cheum-Sampan Rd., Nong Chock, Bangkok 10530 Thailand.
A.T. Animal Clinic 284/3 Village No. 13, Kaset Wisai Roi Et, Thailand.
出版信息
Vet World. 2021 Sep;14(9):2313-2316. doi: 10.14202/vetworld.2021.2313-2316. Epub 2021 Sep 4.
BACKGROUND AND AIM
is an important pathogen causing contagious mastitis in cows that need novel treatment rather than antibiotic therapy. This study aimed to investigate the antimicrobial activity of extracts against isolated from subclinical and clinical mastitis cows using an model.
MATERIALS AND METHODS
pods were extracted using the following solvents: Distilled water, ethanol, acetone, and methanol. The antimicrobial activity of each extract was determined by the disk diffusion method and broth microdilution assay to assess the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
RESULTS
The ethanolic extract of exhibited the largest inhibition zones against (31.05±1.20 mm), followed by the aqueous, methanolic, and acetone extracts, respectively (26.34±1.15, 24.23±0.50, and 22.46±1.53). The ethanolic extract also had a strong inhibitory effect on , with MIC and MBC of 0.13 and 0.52 mg/mL, respectively.
CONCLUSION
This study revealed that is a potential alternative natural antibacterial agent against infection. The antimicrobial activity of is most likely mediated by phytochemical constituents.
背景与目的
是引起奶牛传染性乳腺炎的一种重要病原体，需要新的治疗方法而非抗生素治疗。本研究旨在使用模型研究提取物对从亚临床和临床乳腺炎奶牛分离出的的抗菌活性。
材料与方法
使用以下溶剂提取豆荚：蒸馏水、乙醇、丙酮和甲醇。通过纸片扩散法和肉汤微量稀释法测定每种提取物的抗菌活性，以评估最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
结果
的乙醇提取物对显示出最大的抑菌圈（31.05±1.20毫米），其次分别是水提取物、甲醇提取物和丙酮提取物（26.34±1.15、24.23±0.50和22.46±1.53）。乙醇提取物对也有很强的抑制作用，MIC和MBC分别为0.13和0.52毫克/毫升。
结论
本研究表明是一种潜在的抗感染天然抗菌剂。的抗菌活性很可能由植物化学成分介导。
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