从甜栗木（Mill.）中提取的可水解单宁提取物对泰国黑白花奶牛亚临床乳腺炎致病菌的影响。
Effects of hydrolyzable tannin extract obtained from sweet chestnut wood ( Mill.) against bacteria causing subclinical mastitis in Thai Friesian dairy cows.
作者信息
Prapaiwong Tipwadee, Srakaew Wuttikorn, Wachirapakorn Chalong, Jarassaeng Chaiwat
机构信息
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Division of Theriogenology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
出版信息
Vet World. 2021 Sep;14(9):2427-2433. doi: 10.14202/vetworld.2021.2427-2433. Epub 2021 Sep 20.
BACKGROUND AND AIM
Hydrolyzable tannins are an important group of secondary plant metabolites, which are known for antimicrobial activity. This study aimed to assess the efficiency with which a hydrolyzable tannin extract from sweet chestnut wood ( Mill.) could inhibit mastitis-causing bacteria .
MATERIALS AND METHODS
The negative control used was sterile water, and the positive controls were penicillin and gentamicin. The treatments included five concentrations of hydrolyzable tannins (63, 190, 313, 630, and 940 mg/mL). In cows with subclinical mastitis, the bacteria causing the disease were isolated and identified. Then, the antibacterial activity of the hydrolyzable tannin extract was assessed by the disk diffusion method, by determining the minimum inhibitory concentration (MIC) and by determining the minimum bactericidal concentration (MBC).
RESULTS
Penicillin inhibited (p<0.01) the growth of , , and but could not inhibit (p>0.05) the growth of , , and . However, gentamicin and hydrolyzable tannins could inhibit (p<0.01) all isolated bacteria. Increasing the concentration of hydrolyzable tannin extract resulted in a quadratic increase in the inhibition zone diameter of and and a linear increase in the inhibition zone diameter of , , and . In addition, 630 and 940 mg/mL of hydrolyzable tannin extract showed the highest antibacterial activity against and (p<0.01), while 940 mg/mL concentration had the highest antibacterial activity against (p<0.01). The MIC and MBC of the extract were 27.3-190 mg/mL and 58.8-235 mg/mL, respectively, with the MBC: MIC ratio being 2:1.
CONCLUSION
The antimicrobial activity of the hydrolyzable tannin extract against subclinical mastitis bacteria was comparable to the antibiotics (positive controls) at concentrations over 630 mg/mL. Although these findings are promising, further research is needed to determine whether hydrolyzable tannins could be used to control or prevent subclinical mastitis in dairy cows.
背景与目的
可水解单宁是植物次生代谢产物中的重要一类，以其抗菌活性而闻名。本研究旨在评估甜栗木（ ）中提取的可水解单宁提取物抑制引起乳腺炎的细菌的效率。
材料与方法
所用阴性对照为无菌水，阳性对照为青霉素和庆大霉素。处理包括五种浓度的可水解单宁（63、190、313、630和940 mg/mL）。在患有亚临床乳腺炎的奶牛中，分离并鉴定引起疾病的细菌。然后，通过纸片扩散法、测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）来评估可水解单宁提取物的抗菌活性。
结果
青霉素抑制（p<0.01） 、 和 的生长，但不能抑制（p>0.05） 、 和 的生长。然而，庆大霉素和可水解单宁能抑制（p<0.01）所有分离出的细菌。可水解单宁提取物浓度的增加导致 和 的抑菌圈直径呈二次方增加， 、 和 的抑菌圈直径呈线性增加。此外，630和940 mg/mL的可水解单宁提取物对 和 表现出最高的抗菌活性（p<0.01），而940 mg/mL浓度对 具有最高的抗菌活性（p<0.01）。提取物的MIC和MBC分别为27.3 - 190 mg/mL和58.8 - 235 mg/mL，MBC与MIC的比值为2:1。
结论
可水解单宁提取物对亚临床乳腺炎细菌的抗菌活性在浓度超过630 mg/mL时与抗生素（阳性对照）相当。尽管这些发现很有前景，但还需要进一步研究以确定可水解单宁是否可用于控制或预防奶牛亚临床乳腺炎。
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