Ali Saima, Khan Muhammad Rashid, Sajid Moniba, Zahra Zartash
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
BMC Complement Altern Med. 2018 Jan 31;18(1):43. doi: 10.1186/s12906-018-2114-z.
Parrotiopsis jacquemontiana (Decne) Rehder. is locally used for skin infections and in wound healing. In this study we have evaluated methanol extract of its leaves and derived fractions against the clinical multi-drug resistant bacterial strains.
P. jacquemontiana leaves powder extracted with 95% methanol (PJM) and fractionated in escalating polarity of solvents; n-hexane (PJH), chloroform (PJC), ethyl acetate (PJE), n-butanol (PJB) and the remaining as aqueous fraction (PJA). Clinical as well as environmental 19 bacterial strains and 8 fungal strains were screened for minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Preliminary phytochemical investigation for various phytochemical classes was also carried out.
PJM contained the coumarins, phenols, flavonoids, tannins, alkaloids, glycosides, saponins, sterols, phlobatannins, steroids, phytosterols, triterpenoids, acids, quinones, proteins, vitamin C, betacyanins, oils and resins while anthraquinones, phytosteroids, carbohydrates and anthocyanins were not detected. Disc diffusion assay (1 mg/disc) indicated the sensitivity of all the MDR strains of bacteria with PJM, PJE and PJB, while no inhibition was recorded with PJA. PJH and PJC inhibited the growth of all the strains of Staphylococcus aureus, Pseudomonas aeruginosa and Coagulase negative staphylococci used in this study. Maximum zone of inhibition (35.5 ± 1.32 mm) was obtained with PJM against Staphylococcus lugdenesis MDR (6197). Comparatively lower MIC (8-64 μg/ml) and MBC (32-256 μg/ml) values were recorded for PJM and PJE. In case of fungal strains only PJM, PJE and PJB markedly inhibited the growth and lower MIC (8-128 μg/ml) and MFC (32-512 μg/ml) values were determined for PJM and PJE.
The remarkable inhibition of various bacterial and fungal strains at low doses of the extract/fractions suggested the strong antibacterial, antifungal and anti-candidal potential of P. jacquemontiana leaves.
滇藏棱子芹（Parrotiopsis jacquemontiana (Decne) Rehder.）在当地用于治疗皮肤感染和伤口愈合。在本研究中，我们评估了其叶子的甲醇提取物及其衍生馏分对临床多重耐药细菌菌株的作用。
用95%甲醇提取滇藏棱子芹叶子粉末（PJM），并按溶剂极性递增顺序进行分馏；正己烷（PJH）、氯仿（PJC）、乙酸乙酯（PJE）、正丁醇（PJB），其余为水相馏分（PJA）。对19株临床和环境细菌菌株以及8株真菌菌株进行最小抑菌浓度（MIC）和最小杀菌/杀真菌浓度（MBC/MFC）筛选。还对各种植物化学类别进行了初步植物化学研究。
PJM含有香豆素、酚类、黄酮类、单宁、生物碱、糖苷、皂苷、甾醇、鞣花单宁、类固醇、植物甾醇、三萜类、酸、醌类、蛋白质、维生素C、甜菜花青素、油和树脂，而未检测到蒽醌、植物甾类、碳水化合物和花青素。纸片扩散法（1毫克/片）表明，PJM、PJE和PJB对所有多重耐药细菌菌株均有敏感性，而PJA未记录到抑制作用。PJH和PJC抑制了本研究中使用的所有金黄色葡萄球菌、铜绿假单胞菌和凝固酶阴性葡萄球菌菌株的生长。PJM对卢氏葡萄球菌多重耐药菌（6197）的抑菌圈最大（35.5±1.32毫米）。PJM和PJE的MIC（8 - 64微克/毫升）和MBC（32 - 256微克/毫升）值相对较低。对于真菌菌株，只有PJM、PJE和PJB显著抑制其生长，PJM和PJE的MIC（8 - 128微克/毫升）和MFC（32 - 512微克/毫升）值较低。
提取物/馏分在低剂量下对各种细菌和真菌菌株有显著抑制作用，表明滇藏棱子芹叶子具有很强的抗菌、抗真菌和抗念珠菌潜力。
