Basri Dayang Fredalina, Lew Leong Chen, Muralitharan Raveena Vaidheswary, Nagapan Tava Shelan, Ghazali Ahmad Rohi
Centre for Diagnostic, Therapeutic & Investigative Studies (CODTIS), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Biomedical Science Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Dose Response. 2021 Nov 17;19(4):15593258211047651. doi: 10.1177/15593258211047651. eCollection 2021 Oct-Dec.
Pterostilbene is a potent antioxidant and anti-inflammatory agent. However, its chemopreventive effects via anti-tyrosinase activity and inhibitory effects on melanin content have not been reported previously. Hence, this study aimed to investigate the anti-melanogenic activity of pterostilbene on UVB-irradiated B164A5 mouse melanoma cells. The effects of pterostilbene and resveratrol on cell viability were determined by MTT assay, whereas melanin content and tyrosinase assay were employed to assess melanogenesis activity. Western blot analysis was performed to determine the tyrosinase expression. Based on the MTT assay, the IC value of pterostilbene on UVB-irradiated B164A5 cells was 34.0 ± 3.43 μM, in comparison to resveratrol (>100 μM). Next, 5 and 10 μM pterostilbene showed a significant dose-dependent inhibition ( < .01) of tyrosinase activity in UVB-irradiated B164A5 cells at 37.14 ± 2.71% and 58.36 ± 6.8%, respectively. The findings from the tyrosinase assay also confirmed the downregulation of tyrosinase expression in UVB-irradiated B164A5 cells as measured by Western blot analysis. Finally, 10 μM pterostilbene showed a significantly decreased melanin content ( < .01) in UVB-irradiated B164A5 cells, at 27.34 ± .98 μg/mL. In conclusion, pterostilbene showed anti-melanogenic activity that was 10 times more potent than resveratrol in the UVB-irradiated B164A5 cell.
紫檀芪是一种强效抗氧化剂和抗炎剂。然而，其通过抗酪氨酸酶活性产生的化学预防作用以及对黑色素含量的抑制作用此前尚未见报道。因此，本研究旨在探讨紫檀芪对紫外线B（UVB）照射的B164A5小鼠黑色素瘤细胞的抗黑色素生成活性。通过MTT法测定紫檀芪和白藜芦醇对细胞活力的影响，而采用黑色素含量和酪氨酸酶测定法评估黑色素生成活性。进行蛋白质免疫印迹分析以确定酪氨酸酶表达。基于MTT法，紫檀芪对UVB照射的B164A5细胞的半数抑制浓度（IC值）为34.0±3.43μM，相比之下白藜芦醇的IC值大于100μM。接下来，5μM和10μM紫檀芪对UVB照射的B164A5细胞的酪氨酸酶活性显示出显著的剂量依赖性抑制（P<0.01），分别为37.14±2.71%和58.36±6.8%。酪氨酸酶测定的结果也证实了通过蛋白质免疫印迹分析测得的UVB照射的B164A5细胞中酪氨酸酶表达的下调。最后，10μM紫檀芪使UVB照射的B164A5细胞中的黑色素含量显著降低（P<0.01），为27.34±0.98μg/mL。总之，在UVB照射的B164A5细胞中，紫檀芪显示出比白藜芦醇强10倍的抗黑色素生成活性。
