Nirwana Intan, Munadziroh Elly, Yuliati Anita, Fadhila Azalia Izzah, Wardhana Agung Satria, Shariff Khairul Anuar, Surboyo Meircurius Dwi Condro
Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia.
Bachelor of Dental Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia.
J Oral Biol Craniofac Res. 2022 Jan-Feb;12(1):116-120. doi: 10.1016/j.jobcr.2021.11.008. Epub 2021 Nov 12.
The combination of hydroxyapatite and the herbal extract ellagic acid is expected to accelerate the bone healing process (osteogenesis) due to the extract's anti-inflammatory and antioxidant properties. The osteogenesis process is closely associated with angiogenesis markers, such as fibroblast growth factor 2 (FGF-2), vascular endothelial growth factor (VEGF) and alkali phosphatase (ALP). The objective of this study is to analyse the combination of ellagic acid and hydroxyapatite to promote FGF-2, VEGF and ALP expression as angiogenesis markers in a bone defect model. The research sample comprised 30 male Wistar rats with a defect introduced on the left femur; these were divided into three groups for treatment with ellagic acid and hydroxyapatite, hydroxyapatite and polyethylene glycol (PEG) (control). On days 7 and 14 days after treatment, the Wistar rats were euthanised, and the femoral bone tissue was removed for the immunohistochemical analysis of FGF-2, VEGF and ALP expression. FGF-2 and ALP expression increased in the group treated with ellagic acid and hydroxyapatite on days 7 and 14 post treatment (p < 0.05), and there was an increase in VEGF expression on day 7 post treatment (p < 0.05). The combination of ellagic acid and hydroxyapatite promoted FGF-2, VEGF and ALP expression as angiogenesis markers in the bone defect model.
由于具有抗炎和抗氧化特性，羟基磷灰石与草药提取物鞣花酸的组合有望加速骨愈合过程（骨生成）。骨生成过程与血管生成标志物密切相关，如成纤维细胞生长因子2（FGF-2）、血管内皮生长因子（VEGF）和碱性磷酸酶（ALP）。本研究的目的是分析鞣花酸和羟基磷灰石的组合，以促进骨缺损模型中作为血管生成标志物的FGF-2、VEGF和ALP的表达。研究样本包括30只左股骨有缺损的雄性Wistar大鼠；将它们分为三组，分别用鞣花酸和羟基磷灰石、羟基磷灰石和聚乙二醇（PEG）（对照组）进行治疗。在治疗后第7天和第14天，对Wistar大鼠实施安乐死，并取出股骨组织，用于对FGF-2、VEGF和ALP表达进行免疫组织化学分析。在治疗后第7天和第14天，用鞣花酸和羟基磷灰石治疗的组中FGF-2和ALP表达增加（p<0.05），且在治疗后第7天VEGF表达增加（p<0.05）。在骨缺损模型中，鞣花酸和羟基磷灰石的组合促进了作为血管生成标志物的FGF-2、VEGF和ALP的表达。
