Saraswati Widya, Yahya Azlin Noor, Yonas Yovita, Cindananti Ganiezha, Rahardia Nabiela, Ramadani Rania Rizka, Ambarwati Venny Lusanda, Kusumawardhani Devy Putri, Sutela I Gede Marantika Yogananda, Putri Larasati Kianti, Baskoro Brian Dwi, Pratama Putu Krisnanda, Setiady Dawailatur Rahman
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Restorative Dentistry, Faculty of Dentistry, Universiti Malaya, Malaysia.
Eur J Dent. 2025 Jul;19(3):811-816. doi: 10.1055/s-0044-1793842. Epub 2024 Dec 30.
Calcium carbonate (CaCO), a major inorganic component in bones and teeth, offers potential protection against demineralization. This study investigates the effect of CaCO from shells on the expression of fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGF-β1), and collagen type 1 in the rat dental pulp.
The first maxillary molars of were perforated and subsequently pulp capped with CaCO extracted from shells. The cavities were then filled with glass ionomer cement, while the control group received calcium hydroxide (Ca(OH)). Teeth were extracted after 7 and 14 days of treatment, and the expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp was analyzed using immunohistochemistry staining.
The group treated with CaCO from shells exhibited significantly higher expression of FGF2, TGF-β1, and collagen type 1 in the dental pulp at both 7 and 14 days compared with the group treated with Ca(OH) ( < 0.01).
The application of CaCO derived from shells enhances the proliferative phase in the dental pulp after pulp perforation and perhaps promotes reparative dentine formation.
碳酸钙（CaCO）是骨骼和牙齿中的主要无机成分，具有预防脱矿的潜在作用。本研究调查了来自贝壳的CaCO对大鼠牙髓中成纤维细胞生长因子2（FGF2）、转化生长因子-β1（TGF-β1）和I型胶原表达的影响。
对大鼠的第一上颌磨牙进行穿孔，随后用从贝壳中提取的CaCO覆盖牙髓。然后用玻璃离子水门汀填充窝洞，而对照组接受氢氧化钙（Ca(OH)）。治疗7天和14天后拔牙，采用免疫组织化学染色分析牙髓中FGF2、TGF-β1和I型胶原的表达。
与用Ca(OH)治疗的组相比，用来自贝壳的CaCO治疗的组在7天和14天时牙髓中FGF2、TGF-β1和I型胶原的表达均显著更高（P<0.01）。
应用来自贝壳的CaCO可增强牙髓穿孔后牙髓的增殖期，可能促进修复性牙本质形成。
