Department of Comparative Medicine, The University of Texas MD Anderson Cancer Center, Bastrop, TX, USA.
The University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA.
J Immunol Res. 2021 Nov 8;2021:5318590. doi: 10.1155/2021/5318590. eCollection 2021.
Nonhuman primates are frequently transported to a new location or temporarily relocated within their colony. Both transportation and relocation expose animals to new environments, causing them to undergo a stress response (before adapting). In our NHP colony, the mentioned situations are not infrequent for many reasons, including maintenance. The objective of this study was to determine whether abrupt changes consisting of relocation, housing, separation, and grouping could influence hematological and immunological parameters and thereby functional activity. The current study used squirrel monkeys as a model to investigate the stress-inducing effects of relocation within a facility, while animals acclimated to new situations (physical, housing). A detailed blood analysis revealed significant changes in lymphocytes, triglycerides, total protein, creatinine, and ALT. Flow cytometric analysis of peripheral blood showed reduction in CD3, CD4, and CD8 T cells and monocytes, while B cells and natural killer (NK) cells changed with relocation. Simultaneously, changes in functional activity of immune cells altered proliferative responses and as shown by ELISpot (IFN ). Though the parameters studied are not affected as severely as those in animals transported by road or air, stress responses induced by intrafacility relocation are significant and worth consideration. Our findings indicate that squirrel monkeys mimic the features seen in humans exposed to social stressors and may serve an important model for understanding the mechanisms of stress-induced immune dysfunction in humans.
非人类灵长类动物经常被运送到新的地点或在其群体中临时搬迁。运输和搬迁都会使动物暴露在新的环境中，导致它们经历应激反应（在适应之前）。在我们的非人类灵长类动物群体中，由于许多原因，包括维护，这种情况并不少见。本研究的目的是确定包括搬迁、住房、分离和分组在内的突然变化是否会影响血液学和免疫学参数，从而影响功能活动。本研究使用松鼠猴作为模型，研究了在设施内搬迁引起的应激诱导效应，同时动物适应新环境（身体、住房）。详细的血液分析显示淋巴细胞、甘油三酯、总蛋白、肌酐和 ALT 有显著变化。外周血的流式细胞术分析显示 CD3、CD4 和 CD8 T 细胞和单核细胞减少，而 B 细胞和自然杀伤（NK）细胞随搬迁而变化。同时，免疫细胞功能活动的变化改变了增殖反应，如 ELISpot（IFN）所示。虽然研究的参数没有像通过公路或空运运输的动物那样受到严重影响，但设施内搬迁引起的应激反应是显著的，值得考虑。我们的研究结果表明，松鼠猴模拟了人类暴露于社会应激源时的特征，可能是理解人类应激诱导免疫功能障碍机制的重要模型。
