Shelton Kathryn A, Nehete Bharti P, Chitta Sriram, Williams Lawrence E, Schapiro Steven J, Simmons Joe, Abee Christian R, Nehete Pramod N
Department of Comparative Medicine, MD Anderson Cancer Center, University of Texas, Bastrop, Texas.
Department of Comparative Medicine, MD Anderson Cancer Center, University of Texas, Bastrop, Texas;, Email:
J Am Assoc Lab Anim Sci. 2019 Nov 1;58(6):774-782. doi: 10.30802/AALAS-JAALAS-19-000007. Epub 2019 Oct 11.
NHP are a small, but critical, portion of the animals studied in research laboratories. Many NHP are imported or raised at one facility and subsequently moved to another facility for research purposes. To improve our understanding of the effects of transportation and relocation on the NHP immune system, to minimize potential confounds associated with relocation, and to maximize study validity, we examined the phenotype and function of PBMC in cynomolgus macaques () that were transported approximately 200 miles by road from one facility to another. We evaluated the phenotype of lymphocyte subsets through flow cytometry, mitogen-specific immune responses of PBMC in vitro, and plasma levels of circulating cytokines before transportation, at approximately 24 h after arrival (day 2), and after 30 d of acclimation. Analyses of blood samples revealed that the CD3 and CD4 T-cell counts increased significantly, whereas NK, NKT, and CD14 CD16 nonclassical monocyte subsets were decreased significantly on day 2 after relocation compared with baseline. We also noted significantly increased immune cell function as indicated by mitogen-specific proliferative responses and by IFNγ levels on day 2 compared with baseline. After 30 d of acclimation, peripheral blood CD4 T-cells and monocyte counts were higher than baseline, whereas B-cell numbers were lower. The mitogen-induced responses to LPS and IFNγ production after stimulation with pokeweed mitogen or phytohemagglutinin remained significantly different from baseline. In conclusion, the effects of transportation and relocation on immune parameters in cynomolgus monkeys are significant and do not fully return to baseline values even after 30 d of acclimation.
非人类灵长类动物是研究实验室中所研究动物的一小部分，但却是关键部分。许多非人类灵长类动物在一个设施中进口或饲养，随后为了研究目的转移到另一个设施。为了更好地理解运输和重新安置对非人类灵长类动物免疫系统的影响，尽量减少与重新安置相关的潜在混杂因素，并最大限度地提高研究的有效性，我们研究了食蟹猴外周血单核细胞（PBMC）的表型和功能，这些食蟹猴通过公路从一个设施运输到另一个设施，距离约200英里。我们通过流式细胞术评估淋巴细胞亚群的表型、PBMC在体外对丝裂原的特异性免疫反应，以及在运输前、到达后约24小时（第2天）和适应30天后循环细胞因子的血浆水平。血液样本分析显示，与基线相比，重新安置后第2天CD3和CD4 T细胞计数显著增加，而自然杀伤细胞（NK）、自然杀伤T细胞（NKT）和CD14+CD16非经典单核细胞亚群显著减少。我们还注意到，与基线相比，第2天丝裂原特异性增殖反应和IFNγ水平表明免疫细胞功能显著增强。适应30天后，外周血CD4 T细胞和单核细胞计数高于基线，而B细胞数量较低。用脂多糖（LPS）诱导的丝裂原反应以及在用商陆丝裂原或植物血凝素刺激后IFNγ的产生仍与基线有显著差异。总之，运输和重新安置对食蟹猴免疫参数的影响是显著的，即使在适应30天后也不能完全恢复到基线值。
