Department of Comparative Medicine, The University of Texas MD Anderson Cancer Center, Bastrop, Texas, United States of America.
The University of Texas Graduate School of Biomedical Sciences, Houston, Texas, United States of America.
PLoS One. 2021 Feb 26;16(2):e0240705. doi: 10.1371/journal.pone.0240705. eCollection 2021.
In the present study, we have quantified the effects of transport, relocation and acclimate/adapt to their new surroundings on female squirrel monkey. These responses are measured in blood samples obtained from squirrel monkeys, at different time points relative to their relocation from their old home to their new home. A group of squirrel monkeys we transported, by truck, for approximately 10 hours. Peripheral blood mononuclear cells (PBMCs) were assayed in order to evaluate the phenotype of lymphocyte subsets by flow, mitogen-specific immune responses of PBMCs in vitro, and levels of cytokines at various time points including immediately before transport, immediately upon arrival, and after approximately 150 days of acclimation. We observed significant changes in T cells and subsets, NK and B cells (CD4+, CD8+, CD4+/CD8+, CD16+, and CD20+). Mitogen specific (e.g. PHA, PWM and LPS) proliferation responses, IFN-γ by ELISPOT assay, and cytokines (IL-2, IL-4 and VEGF) significant changes were observed. Changes seen in the serum chemistry measurements mostly complement those seen in the hematology data. The specific goal was to empirically assess the effects of relocation stress in squirrel monkeys in terms of changes in the numbers and functions of various leukocyte subsets in the blood and the amount of time required for acclimating to their new environment. Such data will help to determine when newly arrived animals become available for use in research studies.
在本研究中，我们量化了运输、重新安置以及适应新环境对雌性松鼠猴的影响。这些反应是通过从松鼠猴身上获得的血液样本测量的，这些样本是在它们从旧家搬迁到新家的不同时间点采集的。我们通过卡车运输了一组松鼠猴，大约需要 10 个小时。为了评估淋巴细胞亚群的表型，我们检测了外周血单核细胞（PBMC），通过流式细胞术检测 PBMC 的有丝分裂原特异性免疫反应，以及在不同时间点（包括运输前、到达时和适应后约 150 天）的细胞因子水平。我们观察到 T 细胞和亚群、NK 和 B 细胞（CD4+、CD8+、CD4+/CD8+、CD16+和 CD20+）发生了显著变化。有丝分裂原特异性（如 PHA、PWM 和 LPS）增殖反应、ELISPOT 测定的 IFN-γ以及细胞因子（IL-2、IL-4 和 VEGF）水平发生了显著变化。血清化学测量中的变化大多与血液学数据中的变化相补充。具体目标是根据血液中各种白细胞亚群数量和功能的变化以及适应新环境所需的时间，从经验上评估松鼠猴重新安置应激的影响。这些数据将有助于确定新到达的动物何时可用于研究。
