Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, Jiangxi, China.
Department of Anesthesiology, Guiping People's Hospital, Guigping, 537200, Guangxi, China.
Curr Stem Cell Res Ther. 2022;17(8):815-824. doi: 10.2174/1574888X16666211129121414.
Human adipose-derived stem cells (hASCs) play an important role in regenerative medicine.
Exploring the mechanism of Rg1 in the promotion of the proliferation and adipogenic differentiation of hASCs is important in regenerative medicine research.
To observe ginsenoside Rg1 in promoting the proliferation and adipogenic differentiation of hASCs, Rg1 medium at different concentrations was established and tested using the cell counting kit-8 (CCK-8) assay, oil red O staining, alizarin red, and alcian blue. Compared to the control, differentially expressed genes (DEGs) were screened via DEG analysis, which was carried out in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. To explore the relationship among mRNA, long non-coding RNA (lncRNA) and microRNA (miRNA), we constructed a competing endogenous RNA (ceRNA) network.
In this study, Rg1 was observed to promote the proliferation and adipogenic differentiation of hASCs. Additionally, enriched BPs and KEGG pathways may be involved in the promotion process, where FXR1 and Lnc-GAS5-AS1 were found to be regulatory factors. The regulatory network suggested that Rg1 could regulate the adipocytokine signaling pathway and IL-17 signaling pathway via FXR1 and Lnc-GAS5-AS1, which served as the mechanism encompassing the promotion of Rg1 on the proliferation and adipogenic differentiation of hASCs.
A comprehensive transcriptional regulatory network related to the promotion ability of Rg1 was constructed, revealing mechanisms regarding Rg1's promotion of the proliferation and adipogenic differentiation of hASCs. The present study provides a theoretical basis for optimizing the function of hASCs.
人脂肪间充质干细胞（hASCs）在再生医学中起着重要作用。
探讨 Rg1 促进 hASCs 增殖和脂肪向分化的机制，对于再生医学研究具有重要意义。
为了观察人参皂苷 Rg1 对 hASCs 增殖和脂肪向分化的促进作用，建立了不同浓度的 Rg1 培养基，并用细胞计数试剂盒-8（CCK-8）检测、油红 O 染色、茜素红和阿利新蓝进行检测。通过差异基因分析（DEG 分析）筛选出差异表达基因（DEGs），并进行基因本体（GO）和京都基因与基因组百科全书（KEGG）通路富集分析。为了探讨 mRNA、长链非编码 RNA（lncRNA）和 microRNA（miRNA）之间的关系，构建了竞争内源性 RNA（ceRNA）网络。
本研究观察到 Rg1 促进 hASCs 的增殖和脂肪向分化。此外，富集的 BPs 和 KEGG 通路可能参与了促进过程，其中 FXR1 和 Lnc-GAS5-AS1 被发现是调节因子。调节网络表明，Rg1 可以通过 FXR1 和 Lnc-GAS5-AS1 调节脂肪细胞因子信号通路和 IL-17 信号通路，这是 Rg1 促进 hASCs 增殖和脂肪向分化的机制。
构建了一个与 Rg1 促进能力相关的综合转录调控网络，揭示了 Rg1 促进 hASCs 增殖和脂肪向分化的机制。本研究为优化 hASCs 的功能提供了理论基础。
