1 Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
2 Department of Breast and Thyroid Surgery, The Fifth Affiliated Hospital of Guangxi Medical University &The First People's Hospital of Nanning, Nanning, China.
Cell Transplant. 2019 Mar;28(3):286-295. doi: 10.1177/0963689719825615. Epub 2019 Jan 24.
Augmenting the biological function of adipose-derived stromal cells (ASCs) is a promising approach to promoting tissue remodeling in regenerative medicine. Here, we examined the effect of ginsenoside Rg1 on the paracrine activity and adipogenic differentiation capacity of human breast ASCs (hbASCs) in vitro. hbASCs were isolated and characterized in terms of stromal cell surface markers and multipotency. Third-passage hbASCs were cultured in basic media only or basic media containing different concentrations of G-Rg1 (0.1-100 μM). Cell proliferation was assessed by CCK-8 assay. Paracrine activity was assessed using ELISA. Gene expression was measured by qRT-PCR. Adipogenic differentiation capacity was evaluated by Oil red O staining. We found that hbASCs differentiated into adipocytes, osteoblasts, and chondrocytes in appropriate induction culture medium. hbASCs showed expression of CD29, CD44, CD49d, CD73, CD90, CD105, and CD133 but not CD31 and CD45 surface markers. G-Rg1 increased hbASC proliferation and adipogenic differentiation capacity at lower concentrations (0.1-1 μM) and had the opposite effects at higher concentrations (10-100 μM), while enhanced paracrine activity was observed in all experimental groups compared with control group, and the activation effect of lower concentration G-Rg1 was greater than at higher concentration. These results indicate that G-Rg1 can enhance the proliferation, paracrine activity, and adipogenic differentiation capacity of hbASCs within a certain concentration range. Therefore, the use of G-Rg1 may be beneficial to ASC-assisted fat graft regeneration and soft tissue engineering.
增强脂肪来源基质细胞（ASCs）的生物学功能是促进再生医学中组织重塑的一种有前途的方法。在这里，我们研究了人参皂苷 Rg1 对体外人乳腺 ASC（hbASC）旁分泌活性和脂肪生成分化能力的影响。hbASC 从组织中分离出来并通过基质细胞表面标志物和多能性进行了特征描述。将第三代 hbASC 仅在基础培养基中或含有不同浓度 G-Rg1（0.1-100 μM）的基础培养基中培养。通过 CCK-8 测定法评估细胞增殖。通过 ELISA 评估旁分泌活性。通过 qRT-PCR 测量基因表达。通过油红 O 染色评估脂肪生成分化能力。我们发现 hbASC 在适当的诱导培养基中分化为脂肪细胞、成骨细胞和成软骨细胞。hbASC 表现出 CD29、CD44、CD49d、CD73、CD90、CD105 和 CD133 的表达，但不表达 CD31 和 CD45 表面标志物。G-Rg1 在较低浓度（0.1-1 μM）下增加 hbASC 的增殖和脂肪生成分化能力，而在较高浓度（10-100 μM）下则产生相反的效果，与对照组相比，所有实验组的旁分泌活性均增强，低浓度 G-Rg1 的激活作用大于高浓度。这些结果表明，G-Rg1 可以在一定浓度范围内增强 hbASC 的增殖、旁分泌活性和脂肪生成分化能力。因此，使用 G-Rg1 可能有益于 ASC 辅助脂肪移植再生和软组织工程。
