通过自体细胞毒性T淋巴细胞克隆检测到的人类转移性黑色素瘤克隆的异质性。
Heterogeneity of clones from a human metastatic melanoma detected by autologous cytotoxic T lymphocyte clones.
作者信息
Anichini A, Fossati G, Parmiani G
出版信息
J Exp Med. 1986 Jan 1;163(1):215-20. doi: 10.1084/jem.163.1.215.
Abstract
The possibility that a single human tumor may be composed of an heterogeneous population of cells with respect to susceptibility to lysis by autologous CTL clones was investigated by testing six cytolytic clones derived by micromanipulation against the autologous metastatic melanoma, Me28, and against 31 clones derived from Me28 by cloning in soft agar. Highly significant differences in the lysis of many tumor clones were observed by three of the CTL effectors in comparison with the cytotoxicity achieved on Me28. These results indicate that cloned cellular reagents can detect heterogeneity among cells isolated from the same melanoma, and suggest that the target determinants recognized on the autologous tumor might be differentially expressed on different neoplastic cells.
摘要
通过对6个经显微操作获得的细胞溶解克隆针对自体转移性黑色素瘤Me28以及针对通过软琼脂克隆从Me28获得的31个克隆进行检测，研究了单个人类肿瘤可能由对自体CTL克隆溶解敏感性不同的异质性细胞群体组成的可能性。与对Me28所实现的细胞毒性相比，3个CTL效应细胞在许多肿瘤克隆的溶解方面观察到了高度显著的差异。这些结果表明，克隆的细胞试剂能够检测从同一黑色素瘤分离出的细胞之间的异质性，并提示在自体肿瘤上识别的靶标决定簇可能在不同的肿瘤细胞上有差异地表达。
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