Department of Microbial and Environmental Biotechnology, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Department of Animal Genetics and Breeding, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Reprod Domest Anim. 2022 Mar;57(3):284-291. doi: 10.1111/rda.14058. Epub 2021 Dec 5.
Small heat shock protein B1 (HspB1) has been reported to play an essential role in thermotolerance. This study aimed to determine a correlation (if any) between HspB1 expression and age at first egg lay (puberty) in a native cross-layer poultry of Punjab under heat stress. Forty native cross-layer birds were reared in two different seasons, viz. summer (THI was more than 27), classified as the heat-stressed group (n = 20) and winter season (THI was less than 21), classified as the control group (n = 20). Blood was collected from both the groups of birds in their 15 week of age and at puberty. Serum catalase and superoxide dismutase activities, reduced glutathione and corticosterone concentration and lipid peroxidation were measured to assess the oxidative stress in both the groups of birds. The serum antioxidants significantly decreased whilst corticosterone levels and lipid peroxidation significantly elevated in birds in response to summer heat stress. Moreover, in summer season, the activities of the antioxidant enzymes further decreased and lipid peroxidation further increased significantly in birds from their pre-pubertal stage to puberty, which was not observed during the winter season. A clone of chicken HspB1 in BL21 (DE3) cells was revived, and recombinant HspB1 was purified using Ni-NTA agarose column. Serum HspB1 concentration was estimated in different groups of birds by indirect ELISA that has been standardized using the recombinant chicken HspB1. Compared to the control, birds under heat stress had significantly higher serum HspB1 levels. The delay in puberty of all the heat-stressed birds was significantly associated with the increase in their serum HspB1 levels. Taken together, the expression of HspB1 was found to be associated with age at puberty in the native cross poultry layers of Punjab.
小分子热休克蛋白 B1（HspB1）已被报道在耐热性中发挥重要作用。本研究旨在确定在热应激下旁遮普邦本地杂交禽类中 HspB1 表达与首次产卵（青春期）年龄之间是否存在相关性。40 只本地杂交禽类在两个不同季节饲养，即夏季（THI 超过 27），归类为热应激组（n=20）和冬季（THI 低于 21），归类为对照组（n=20）。在 15 周龄和青春期时从两组禽类中采集血液。测量血清过氧化氢酶和超氧化物歧化酶活性、还原型谷胱甘肽和皮质酮浓度以及脂质过氧化，以评估两组禽类的氧化应激。血清抗氧化剂显著降低，而皮质酮水平和脂质过氧化显著升高，以应对夏季热应激。此外，在夏季，抗氧化酶的活性进一步降低，脂质过氧化进一步显著增加，从禽类的青春期前阶段到青春期，而在冬季则没有观察到这种情况。在 BL21（DE3）细胞中复活了鸡 HspB1 的克隆，并使用 Ni-NTA 琼脂糖柱纯化重组 HspB1。通过间接 ELISA 测定不同组禽类的血清 HspB1 浓度，该 ELISA 已使用重组鸡 HspB1 进行标准化。与对照组相比，热应激组的禽类血清 HspB1 水平显著升高。所有热应激鸟类的青春期延迟与血清 HspB1 水平的升高显著相关。总之，HspB1 的表达与旁遮普邦本地杂交禽类的青春期年龄有关。
