Department of Microbial and Environmental Biotechnology, College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141004, India.
Department of Animal Genetics and Breeding, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141004, India.
Int J Biometeorol. 2020 Jul;64(7):1133-1143. doi: 10.1007/s00484-020-01887-z. Epub 2020 Mar 9.
Heat shock proteins (Hsp) aid in protein folding and also to combat stress in all cellular organisms. HspB1 is a member of the small HSP family that has a significant role in thermo-tolerance. In this study, we aimed to determine the relationship (if any) between age at sexual maturity of layer poultry (Rhode Island Red and Punjab Red) and HspB1 expression both at mRNA and protein levels under heat stress. The mRNA expression of hspB1 was checked by real-time PCR. Delay in sexual maturity of the birds was found to be directly associated with the hspB1 mRNA expression in both the bird varieties under heat stress. No significant regression (association) of hspB1 mRNA expression with age at sexual maturity was observed in case of control, non-heat stressed birds. The serum levels of HspB1 were measured by indirect ELISA, using recombinant HspB1 that was expressed using pET-32b(+) vector in BL21(DE3) cells. Serum HspB1 concentration increased significantly (p ≤ 0.001) in heat-stressed birds as compared with control ones. A significant association was found between the increase in serum HspB1 concentration and delay in sexual maturity of all the birds under heat stress while no such association was found in control birds. In conclusion, HspB1 mRNA and protein expression were found to be associated with age at sexual maturity in Punjab Red and RIR layers under heat stress.
热休克蛋白（Hsp）有助于蛋白质折叠，并帮助所有细胞生物应对压力。HspB1 是小分子 HSP 家族的一员，在耐热性方面发挥着重要作用。在这项研究中，我们旨在确定层状家禽（罗得岛红和旁遮普红）的性成熟年龄与热应激下 HspB1 表达（如果有的话）之间的关系。通过实时 PCR 检查 hspB1 的 mRNA 表达。发现禽类性成熟的延迟与两种鸟类在热应激下 hspB1 mRNA 表达直接相关。在非热应激的对照鸟类中，hspB1 mRNA 表达与性成熟年龄之间没有观察到显著的回归（关联）。使用在 BL21(DE3) 细胞中使用 pET-32b(+) 载体表达的重组 HspB1 通过间接 ELISA 测量血清 HspB1 水平。与对照组相比，热应激鸟类的血清 HspB1 浓度显着增加（p≤0.001）。在热应激下，所有鸟类的血清 HspB1 浓度增加与性成熟延迟之间存在显著关联，而在对照组鸟类中则没有发现这种关联。总之，在热应激下，旁遮普红和 RIR 层状鸡的 HspB1 mRNA 和蛋白质表达与性成熟年龄有关。
