Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, P. R. China.
Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, Shandong, P. R. China.
Bioengineered. 2021 Dec;12(2):10891-10904. doi: 10.1080/21655979.2021.1994908.
Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the levels of immune/stromal components and the proportion of tumor-infiltrating immune cells (TIICs) were determined in 611 ccRCC samples using the ESTIMATE and CIBERSORT analytical tools. Subsequently, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was identified by univariate Cox regression analysis, protein-protein interaction (PPI) networks and clinical survival analysis to be associated with ccRCC prognosis. At the same time, the abundance of HSD11B1 increased significantly in ccRCC was verified by western blotting, RT‑qPCR and immunostaining analysis. Furthermore, Gene Set Enrichment Analysis (GSEA) and TME suggested that HSD11B1 was involved in TME immune-related status. Taken together, the results of the present study demonstrated that HSD11B1 is a potential prognostic biomarker associated with immune cell infiltration in ccRCC.
透明细胞肾细胞癌（ccRCC）是全球最常见的恶性肿瘤之一。ccRCC 的临床治疗与肿瘤微环境（TME）密切相关。鉴定 ccRCC 的潜在标志物对于改善预后很重要。因此，在本研究中，使用 ESTIMATE 和 CIBERSORT 分析工具，在 611 个 ccRCC 样本中测定了免疫/基质成分的水平和肿瘤浸润免疫细胞（TIIC）的比例。随后，通过单因素 Cox 回归分析、蛋白质-蛋白质相互作用（PPI）网络和临床生存分析鉴定到羟类固醇 11-β 脱氢酶-1（HSD11B1）与 ccRCC 预后相关。同时，通过 Western blot、RT-qPCR 和免疫组化分析验证了 HSD11B1 在 ccRCC 中的丰度显著增加。此外，基因集富集分析（GSEA）和 TME 表明 HSD11B1 参与了 TME 免疫相关状态。综上所述，本研究结果表明 HSD11B1 是与 ccRCC 免疫细胞浸润相关的潜在预后生物标志物。
