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sp. nov.,分离自北极冻原土壤,对 和 进行了描述的修正。

sp. nov., isolated from Arctic tundra soil, and emended descriptions of , and .

机构信息

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

College of Science, Tibet University, Tibet 850000, PR China.

出版信息

Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005103.

Abstract

Strain Q3-56, isolated from Arctic tundra soil, was found to be a Gram-stain-negative, yellow-pigmented, oxidase- and catalase-positive, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain Q3-56 grew optimally at pH 7.0 and 28 °C. The strain could tolerate up to 1 % (w/v) NaCl with optimum growth in the absence of NaCl. The strain was not sensitive to oxacillin and ceftazidime. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q3-56 belonged to the genus . Strain Q3-56 showed the highest sequence similarities to T17 (96.58 %), Gsoil 043 (96.50 %), NS28 (96.43 %) and QTA69 (96.43 %). The predominant respiratory isoprenoid quinone was identified as MK-7, The polar lipid profile of strain Q3-56 was found to contain one phosphatidylethanolamine, three unidentified aminolipids, three unidentified lipids and one unidentified phospholipid. The G+C content of the genomic DNA was determined to be 49.1 mol%. The main fatty acids were summed feature 3 (comprising C 7/C 6), iso-C, C 5 and iso-C 3-OH. On the basis of the evidence presented in this study, a novel species of the genus , sp. nov., is proposed, with the type strain Q3-56 (=CCTCC AB 2019271=KCTC 72739). Emended descriptions of , and are also provided.

摘要

Q3-56 菌株,从北极苔原生态土壤中分离得到,是革兰氏阴性、黄色素、氧化酶和过氧化氢酶阳性、非运动、非孢子形成、杆状和需氧细菌。Q3-56 菌株在 pH7.0 和 28°C 下生长最佳。该菌株可以耐受高达 1%(w/v)的 NaCl,在没有 NaCl 的情况下生长最佳。该菌株对苯唑西林和头孢他啶不敏感。基于 16S rRNA 基因序列的系统发育分析表明,菌株 Q3-56 属于 属。菌株 Q3-56 与 T17(96.58%)、Gsoil 043(96.50%)、NS28(96.43%)和 QTA69(96.43%)的序列相似度最高。主要的呼吸异戊二烯醌被鉴定为 MK-7。菌株 Q3-56 的极性脂谱中含有一个磷脂酰乙醇胺、三个未鉴定的氨基脂、三个未鉴定的脂和一个未鉴定的磷脂。基因组 DNA 的 G+C 含量为 49.1mol%。主要脂肪酸为特征脂肪酸 3(包含 C7/C6)、iso-C、C5 和 iso-C3-OH。根据本研究提供的证据,提出了一个新的属 种,即 sp. nov.,其模式菌株为 Q3-56(=CCTCC AB 2019271=KCTC 72739)。还对 、 和 的描述进行了修正。

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