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新种,新种,以及新种,从青藏高原土壤中分离得到。

sp. nov., sp. nov., and sp. nov., isolated from soil of the Qinghai-Tibetan Plateau.

机构信息

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

出版信息

Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005747.

Abstract

Six novel bacterial strains, designated CY22, CY357, LJ419, LJ53, CY399 and CY107 were isolated from soil samples collected from the Qinghai-Tibetan Plateau, PR China. Cells were aerobic, rod-shaped, yellow-pigmented, catalase- and oxidase-positive, Gram-stain-negative, non-motile and non-spore-forming. All strains were psychrotolerant and could grow at 0 °C. The results of phylogenetic and phylogenomic analyses, based on 16S rRNA gene sequences and core genomic genes, indicated that the three strain pairs (CY22/CY357, LJ419/LJ53 and CY399/CY107) were closely related to members of the genus and clustered tightly with two species with validly published names, 12116 and BZ26. Values of digital DNA-DNA hybridization between genome sequences of the isolates and other strains from the GenBank database in the genus were far below the 70.0 % threshold. The genomic DNA G+C content of these six strains ranged from 45.2 to 45.8 %. The major cellular fatty acids of all six strains were -C and summed feature 3 (comprising C 7 and/or C 6). MK-7 was the only respiratory quinone, and phosphatidylethanolamine was the predominant polar lipid for strains CY22, LJ419 and CY399. On the basis of the phenotypic, phylogenetic and genomic evidence presented, these six strains represent three novel members of the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are CY22 (= GDMCC 1.3045 = KCTC 92299), LJ419 (= GDMCC 1.2872 = JCM 33794) and CY399 (= GDMCC 1.3052 = KCTC 92306), respectively.

摘要

从中国青藏高原土壤样本中分离到的 6 株新的细菌菌株,分别命名为 CY22、CY357、LJ419、LJ53、CY399 和 CY107。细胞呈需氧、杆状、黄色素、过氧化氢酶和氧化酶阳性、革兰氏阴性、非运动和非孢子形成。所有菌株均为嗜冷菌,可在 0°C 下生长。基于 16S rRNA 基因序列和核心基因组基因的系统发育和系统基因组分析结果表明,这三对菌株(CY22/CY357、LJ419/LJ53 和 CY399/CY107)与属内的成员密切相关,并与两个具有有效发表名称的种聚在一起,即 12116 和 BZ26。这些分离株与 GenBank 数据库中属内其他菌株的基因组 DNA-DNA 杂交值远低于 70.0%的阈值。这 6 株菌的基因组 DNA G+C 含量范围为 45.2%至 45.8%。所有 6 株菌的主要细胞脂肪酸均为 -C 和总和特征 3(包括 C 7 和/或 C 6)。MK-7 是唯一的呼吸醌,磷脂酰乙醇胺是菌株 CY22、LJ419 和 CY399 的主要极性脂。基于表型、系统发育和基因组证据,这 6 株菌代表属的 3 个新成员,分别提议将它们命名为 sp. nov.、 sp. nov. 和 sp. nov.。模式菌株分别为 CY22(=GDMCC 1.3045=KCTC 92299)、LJ419(=GDMCC 1.2872=JCM 33794)和 CY399(=GDMCC 1.3052=KCTC 92306)。

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