College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Huiqun fertilizer co Ltd., Horqin Left Middle Banner, Inner Mongolia Autanomous Region 029315, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2281-2287. doi: 10.1099/ijsem.0.004030. Epub 2020 Feb 10.
A Gram-reaction-negative, yellow-pigmented, rod-shaped, aerobic, non-motile, non-spore-forming bacterium, designated strain QTA69, was isolated from a subsurface sediment sample collected at the Qiangtang basin, Qinghai-Tibetan Plateau, PR China. Cells were catalase-positive and oxidase-negative. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QTA69 was a member of the genus and was closely related to , and with sequence similarities from 97.90 % to 96.85 %. Strain QTA69 grew at 4-35 °C, and the optimum temperature was 25-28 °C. It grew at the pH range of 6.0-9.0 (optimum, pH 7.0-8.0) and its NaCl tolerance was 0-2.0 % (optimum, 0-1.0 %). The major cellular fatty acids were summed feature 3 (iso-C 2-OH and C ω6/Cω7), iso-C and Cω5. The major respiratory quinone was MK-7 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 8.41 Mbp and a G+C content of 46.87 mol%. Based on whole genome average nucleotide identity values, phenotypic data, phylogenetic data and genotypic data, strain QTA69 represents a novel species of genus , for which the name sp. nov is proposed. The type strain is QTA69 (=CGMCC 1.13688=KCTC 72024).
一株革兰氏反应阴性、黄色着色、杆状、需氧、非运动、非孢子形成的细菌,被命名为 QTA69 菌株,从中国青藏高原羌塘盆地的地下沉积物样本中分离得到。细胞呈过氧化氢酶阳性和氧化酶阴性。基于 16S rRNA 基因序列的系统发育分析表明,菌株 QTA69 是属的一个成员,与 、 和 最为密切相关,序列相似度为 97.90%至 96.85%。QTA69 菌株在 4-35°C 下生长,最适温度为 25-28°C。它在 pH 值为 6.0-9.0 的范围内生长(最适 pH 值为 7.0-8.0),能耐受 0-2.0%的 NaCl(最适为 0-1.0%)。主要细胞脂肪酸为 summed feature 3(iso-C 2-OH 和 C ω6/Cω7)、iso-C 和 Cω5。主要呼吸醌为 MK-7,主要极性脂为磷脂酰乙醇胺。基因组测序揭示其基因组大小为 8.41 Mbp,G+C 含量为 46.87 mol%。基于全基因组平均核苷酸同一性值、表型数据、系统发育数据和基因型数据,菌株 QTA69 代表属的一个新种,建议将其命名为 sp. nov。其模式菌株是 QTA69(=CGMCC 1.13688=KCTC 72024)。