College of Horticulture, South China Agricultural University, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, PR China.
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005118.
A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as strain B61, was isolated from rhizosphere soil of banana collected from Dongguan, Guangdong Province, PR China. Growth occurred at 15-40 °C, within a pH range of pH 6.0-9.0. Results of 16S rRNA gene sequence similarity and phylogenetic analyses showed that strain B61 was most closely related to '' KACC 21303 (98.9 %) and DSM 2588 (98.8 %). The genome size was 7.6 Mb with a G+C content of 45.2 mol%. The genome-inferred average nucleotide identity values between strain B61 and two closely related strains were 79.2 and 79.3 %, respectively, with corresponding digital DNA-DNA hybridization values of 22.3 and 22.6 %. The major fatty acids of the novel strain were iso-C, C 5 and iso-C 3-OH and the sole respiratory quinone was menaquinone 7 (MK-7). The polar lipids consisted of phosphatidylethanolamine, five unidentified aminolipids, four unidentified glycolipids and six unidentified lipids. The phenotypic and phylogenetic results clearly supported that strain B61 represents a novel species of the genus , for which the name , sp. nov. is proposed, with the type strain B61 (=GDMCC 1.2608=KCTC 82856).
一株革兰氏阴性、需氧、杆状细菌，被命名为 B61 菌株，从中国广东省东莞市采集的香蕉根际土壤中分离得到。该菌在 15-40°C、pH 值 6.0-9.0 的范围内生长。16S rRNA 基因序列相似性和系统发育分析结果表明，B61 菌株与 '' KACC 21303（98.9%）和 DSM 2588（98.8%）最为密切相关。基因组大小为 7.6 Mb，G+C 含量为 45.2 mol%。B61 菌株与两个密切相关菌株的全基因组平均核苷酸同一性值分别为 79.2%和 79.3%，相应的数字 DNA-DNA 杂交值分别为 22.3%和 22.6%。该新菌株的主要脂肪酸为 iso-C、C5 和 iso-C3-OH，唯一的呼吸醌为menaquinone 7（MK-7）。极性脂由磷脂酰乙醇胺、五种未鉴定的胺脂、四种未鉴定的糖脂和六种未鉴定的脂组成。表型和系统发育结果清楚地表明，B61 菌株代表了一个新的属种，命名为 ，sp. nov.，其模式菌株为 B61（=GDMCC 1.2608=KCTC 82856）。
