Zou Yuanyuan, Zhang Xiaoxia, Song Haozhi, Liu Yang, Cheng Qi
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Agricultural Resources and Regional Planning Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1179-1184. doi: 10.1099/ijsem.0.003292. Epub 2019 Feb 18.
A Gram-stain-negative, non-flagellated, rod-shaped bacterium, designated strain T22, was isolated from rhizosphere soil of Alhagi sparsifolia, collected from Xinjiang, China. Its major fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0-3OH, summed feature 1 (C13 : 0 3-OH/iso-C15 : 1 H) and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, two aminolipids and four unidentified lipids. The DNA G+C content of the type strain was 53.4 mol%. According to phylogenetic analysis based on 16S rRNA gene sequences, strain T22 was related most closely to Chitinophaga barathri YLT18 (=CCTCC AB 2015054) with similarity of 97.7 %. However, strain T22 was clearly distinguished from Chitinophaga barathri YLT18 using genome-to-genome distance and average nucleotide identity value calculation, as well as a range of physiological and biochemical characteristics comparisons. It is obvious from the genotypic and phenotypic data that strain T22 represents a novel species of the genus Chitinophaga, for which the name Chitinophaga alhagiae sp. nov., is proposed. The type strain is T22 (=ACCC 60125=KCTC 62518).
从中国新疆采集的骆驼刺根际土壤中分离出一株革兰氏染色阴性、无鞭毛、杆状细菌，命名为T22菌株。其主要脂肪酸（>5%）为异C15:0、C16:1ω5c、异C17:0-3OH、特征性总和1（C13:0 3-OH/异C15:1 H）和特征性总和3（C16:1ω6c/C16:1ω7c）。主要呼吸醌为MK-7。主要极性脂为磷脂酰乙醇胺、两种氨基脂和四种未鉴定的脂。模式菌株的DNA G+C含量为53.4 mol%。基于16S rRNA基因序列的系统发育分析表明，T22菌株与巴氏几丁质噬菌YLT18（=CCTCC AB 2015054）关系最为密切，相似度为97.7%。然而，通过基因组到基因组距离和平均核苷酸同一性值计算以及一系列生理生化特征比较，T22菌株与巴氏几丁质噬菌YLT18明显区分开来。从基因型和表型数据可以明显看出，T22菌株代表几丁质噬菌属的一个新物种，为此提出名为巴氏几丁质噬菌（Chitinophaga alhagiae sp. nov.）。模式菌株为T22（=ACCC 60125=KCTC 62518）。
