Ke Zhuang, Yang Mu-Ji, Jia Wei-Bin, Mu Yang, Li Jian-Long, Chen Dian, Chen Kai, Jiang Jian-Dong
1Department of Microbiology, Key Lab of Environmental Microbiology for Agriculture, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
2Jiangsu Key Lab for Solid Organic Waste Utilization, 210095 Nanjing, PR China.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3452-3457. doi: 10.1099/ijsem.0.003006. Epub 2018 Sep 12.
A Gram-stain-negative bacterium, designated LY-1, was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1 were strictly aerobic, non-motile and rod-shaped. Strain LY-1 grew optimally at pH 7.0 and at 30-35 °C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1 was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2 (97.5 %) and lower (<97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1 possessed menaquinone-7 as the major isoprenoid quinone; and iso-C15 : 0 (46.4 %), C16 : 1ω5c (27.8 %) and iso-C17 : 0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1 consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1 was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value of the draft genomes between strain LY-1 and strain A37T2 were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA-DNA hybridization, strain LY-1 is considered a novel species of the genus Chitinophaga, for which the name Chitinophagaparva sp. nov. (type strain LY-1=CCTCC AB 2018018=KCTC 62444) is proposed.
从中国安徽省阜阳市一家化工厂采集的土壤样本中分离出一株革兰氏阴性菌，命名为LY-1。LY-1菌株的细胞严格需氧，无运动性，呈杆状。LY-1菌株在pH 7.0以及30-35 °C条件下生长最佳。采用多相分类法对其分类地位进行了研究。基于16S rRNA基因序列的系统发育分析表明，LY-1菌株是噬几丁质菌属的成员，与Costaii噬几丁质菌A37T2的序列相似性最高（97.5%），与其他已知噬几丁质菌属物种的序列相似性较低（<97.0%）。化学分类分析表明，LY-1菌株以甲基萘醌-7作为主要类异戊二烯醌；主要脂肪酸为异-C15 : 0（46.4%）、C16 : 1ω5c（27.8%）和异-C17 : 0 3-OH（9.0%）。LY-1菌株的极性脂质由磷脂酰乙醇胺、三种未鉴定的磷酸氨基脂质、一种未鉴定的磷脂、四种未鉴定的脂质、两种未鉴定的氨基脂质和两种未鉴定的糖脂组成。基于全基因组计算，LY-1菌株的基因组DNA G+C含量为52.4 mol%。LY-1菌株与A37T2菌株草图基因组的平均核苷酸同一性和数字DNA-DNA杂交值分别为76.8%和19.8%。基于系统发育和表型特征、化学分类数据以及DNA-DNA杂交结果，LY-1菌株被认为是噬几丁质菌属的一个新物种，为此提出名为微小噬几丁质菌（Chitinophaga parva sp. nov.）（模式菌株LY-1=CCTCC AB 2018018=KCTC 62444）。
