Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States.
LifeNet Health, Virginia Beach, VA, United States.
Bioelectrochemistry. 2022 Apr;144:107981. doi: 10.1016/j.bioelechem.2021.107981. Epub 2021 Oct 29.
Gene therapy has a wide range of applications for various types of pathologies. Viral methods of gene delivery provide high levels of gene expression but have various safety concerns. Non-viral methods are largely known to provide lower levels of expression. We aim to address this issue by using plasmid DNA with smaller backbones to increase gene expression levels when delivered using non-viral methods. In this study we compare gene expression levels between two vectors with firefly luciferase encoding gene insert using liposome complexes and gene electrotransfer as delivery methods. A 2-fold reduction in plasmid vector backbone size, disproportionately enhanced gene expression levels more than 10-fold in rat tenocytes in vitro, and rat myocardium in vivo, while improvements in delivery to the skin were more moderate.
基因治疗在各种类型的病理中具有广泛的应用。病毒基因传递方法可提供高水平的基因表达，但存在各种安全问题。非病毒方法的表达水平通常较低。我们旨在通过使用具有较小骨架的质粒 DNA 来解决这个问题，当使用非病毒方法传递时，提高基因表达水平。在这项研究中，我们比较了两种载体的基因表达水平，这两种载体都使用脂质体复合物和基因电转移作为传递方法，插入萤火虫荧光素酶编码基因。质粒载体骨架大小减少了 2 倍，不成比例地使体外大鼠肌腱细胞和体内大鼠心肌中的基因表达水平提高了 10 多倍，而对皮肤的传递改善则较为温和。
