Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, Henan, China.
Sci Rep. 2021 Nov 30;11(1):23115. doi: 10.1038/s41598-021-02573-9.
To compare stent-induced granulation tissue hyperplasia of bare (SEMS), polyurethane-covered (PU-SEMS) and electrospun nanofibre-covered (EN-SEMS) self-expandable metallic stents in the rabbit trachea. Twenty-seven rabbits were randomly assigned to 3 groups that received SEMS, PU-SEMS or EN-SEMS. Computed tomography and sacrifice were performed as scheduled. Haematoxylin-eosin and Masson's trichrome staining protocols were performed for pathological analysis. The data for tracheal ventilation area ratio, qualitative histological scoring, number of epithelial layers, and thicknesses of papillary projection and submucosa were documented and statistically analysed. All stents were successfully placed under the guidance of fluoroscopy without complications. Post-stenting 3 and 7 days, computed tomography revealed that the fully expandable EN-SEMS was similar to the SEMS and PU-SEMS. The mean stented tissue score in the SEMS group was higher than those of both the PU-SEMS and EN-SEMS groups at 3 days post-stenting. The pathological findings suggested that there was no papillary projection formation 3 days after stent placement. The thickness of papillary projection in the SEMS group was significantly higher than those of the PU-SEMS and EN-SEMS groups at 7 days post-stenting. After stenting 4 weeks, the tracheal ventilation area ratio of SEMS, PU-SEMS and EN-SEMS was 0.214 ± 0.021, 0.453 ± 0.028 and 0.619 ± 0.033, respectively. There were significant between-group differences. In conclusion, the stent-induced granulation tissue formation in EN-SEMS is less severe than that of PU-SEMS and SEMS. EN-SEMS has smaller radial force, and the tracheal ventilation ratio after stent placement better than that of PU-SEMS.
比较裸（SEMS）、聚氨酯覆盖（PU-SEMS）和电纺纳米纤维覆盖（EN-SEMS）自膨式金属支架在兔气管中的支架诱导肉芽组织增生。27 只兔子随机分为 3 组，分别接受 SEMS、PU-SEMS 或 EN-SEMS 治疗。按计划进行计算机断层扫描和处死。进行苏木精-伊红和 Masson 三色染色方案进行病理分析。记录并统计分析气管通气面积比、定性组织学评分、上皮层数以及乳头状突起和黏膜下厚度的数据。所有支架均在透视引导下成功放置，无并发症。支架置入后 3 天和 7 天，计算机断层扫描显示完全可扩张的 EN-SEMS 与 SEMS 和 PU-SEMS 相似。支架置入后 3 天，SEMS 组的平均支架组织评分高于 PU-SEMS 和 EN-SEMS 组。支架置入后 3 天，支架内组织未见乳头状突起形成。支架置入后 7 天，SEMS 组的乳头状突起厚度明显高于 PU-SEMS 和 EN-SEMS 组。支架置入 4 周后，SEMS、PU-SEMS 和 EN-SEMS 的气管通气面积比分别为 0.214±0.021、0.453±0.028 和 0.619±0.033，组间差异有统计学意义。结论，EN-SEMS 支架诱导的肉芽组织形成比 PU-SEMS 和 SEMS 轻。EN-SEMS 的径向力较小，支架置入后气管通气率优于 PU-SEMS。
