Ghasemzadeh Fatemeh, Najafpour Ghasem D, Mohammadi Maedeh
Biotechnology Research Laboratory, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol Iran.
Turk J Chem. 2021 Oct 19;45(5):1454-1462. doi: 10.3906/kim-2104-13. eCollection 2021.
The present study aimed to synthesize ursolic acid-loaded chitosan nanoparticles (UA-Ch-NPs) as an antiinfective agent against 21 isolates. The UA-Ch-NPs were synthesized by a simple method and then characterized by TEM, FTIR, DLS-zeta potential, and XRD analyses. According to the characterization results, highly dispersed spherical nanoparticles with a mean diameter of 258 nm and a zeta potential of + 40.1 mV were developed. The antibacterial properties of UA-Ch-NPs were investigated and their inhibitory effect on biofilm formation was demonstrated by AFM. Finally, the expression levels of icaA and icaD were measured using real-time PCR. Results indicated that the minimum inhibitory concentration (MIC) of UA and UA-Ch-NPs against was 64 and 32 µg/mL, respectively. The treatment of bacterial cells with UA-Ch-NPs significantly decreased the expression of icaA and icaD genes which are engaged in biofilm formation. Our results indicated that UA-Ch-NPs could be a promising material for antibacterial and antibiofilm applications.
本研究旨在合成负载熊果酸的壳聚糖纳米颗粒（UA-Ch-NPs）作为针对21株分离菌的抗感染剂。通过一种简单方法合成了UA-Ch-NPs，然后通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、动态光散射-ζ电位和X射线衍射（XRD）分析对其进行表征。根据表征结果，制备出了平均直径为258 nm、ζ电位为+40.1 mV的高度分散的球形纳米颗粒。研究了UA-Ch-NPs的抗菌性能，并通过原子力显微镜（AFM）证明了其对生物膜形成的抑制作用。最后，使用实时聚合酶链反应（PCR）测量icaA和icaD的表达水平。结果表明，UA和UA-Ch-NPs对……的最低抑菌浓度（MIC）分别为64和32 μg/mL。用UA-Ch-NPs处理细菌细胞显著降低了参与生物膜形成的icaA和icaD基因的表达。我们的结果表明，UA-Ch-NPs可能是用于抗菌和抗生物膜应用的有前景的材料。
