Lee Chia-Ming, Tian Xuejiao, Tsao Chieh, Chen Peilin, Huang Tzyy-Nan, Hsueh Yi-Ping, Chen Bi-Chang
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.
Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan.
Discoveries (Craiova). 2021 Aug 4;9(3):e133. doi: 10.15190/d.2021.12. eCollection 2021 Jul-Sep.
Macro photography allows direct visualization of the enlarged whole mouse brain by a combination of lightsheet illumination and expansion microscopy with single-cell resolution. Taking advantage of the long working distance of a camera lens, we imaged a 3.7 cm thick, transparent, fluorescently-labeled expanded brain. In order to improve 3D sectioning capability, we used lightsheet excitation confined as the depth of field of the camera lens. Using 4x sample expansion and 5x optical magnification, macro photography enables imaging of expanded whole mouse brain with an effective resolution of 300 nm, which provides the subcellular structural information at the organ level.
宏观摄影通过光片照明和具有单细胞分辨率的扩展显微镜相结合，能够直接可视化放大的整个小鼠大脑。利用相机镜头的长工作距离，我们对一个3.7厘米厚、透明、荧光标记的扩展大脑进行了成像。为了提高三维切片能力，我们将光片激发限制在相机镜头的景深范围内。通过4倍的样本扩展和5倍的光学放大，宏观摄影能够以300纳米的有效分辨率对扩展的整个小鼠大脑进行成像，从而在器官水平上提供亚细胞结构信息。
