Department of Pathology, Pingshan District People's Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, Guangdong Province, China.
Bioengineered. 2021 Dec;12(2):10761-10770. doi: 10.1080/21655979.2021.2003130.
The incidence and mortality of breast cancer rank first among all types of female tumors. To improve patients' prognosis with advanced breast cancer, new and more effective targets still need to be explored and identified. Tetraspanin 1 (TSPAN1) is highly expressed in several cancers and affects the progression of these tumors. However, there are few studies focused on its role in breast cancer. Previous study showed that TSPAN1 promoted epithelial-mesenchymal transition (EMT) and metastasis, and whether TSPAN1 could promote breast cancer via regulating EMT needs further study. In this study, we found high TSPAN1 expression in breast cancer tumor samples and cell lines which was confirmed by bioinformation analysis. The ablation of TSPAN1 suppressed the growth, and motility of breast cancer cells. We further found that TSPAN1 affected the EMT and mediated the PI3K/Akt pathway in breast cancer cells. In addition, TSPAN1 depletion suppressed tumor growth of breast cancer in mice. In summary, we thought TSPAN1 suppressed growth and motility of breast cancer via mediating EMT and PI3K/AKT pathway, and could serve as a potential target for treatment of breast cancer.
乳腺癌的发病率和死亡率在所有女性肿瘤中位居第一。为了改善晚期乳腺癌患者的预后，仍需要探索和确定新的、更有效的靶点。四跨膜蛋白 1（TSPAN1）在多种癌症中高表达，影响这些肿瘤的进展。然而，关于其在乳腺癌中的作用的研究较少。先前的研究表明，TSPAN1 促进上皮-间充质转化（EMT）和转移，TSPAN1 是否可以通过调节 EMT 促进乳腺癌还需要进一步研究。在这项研究中，我们通过生物信息分析发现 TSPAN1 在乳腺癌肿瘤样本和细胞系中高表达。TSPAN1 的缺失抑制了乳腺癌细胞的生长和迁移。我们进一步发现 TSPAN1 影响了乳腺癌细胞的 EMT，并介导了 PI3K/Akt 通路。此外，TSPAN1 耗竭抑制了乳腺癌在小鼠中的肿瘤生长。总之，我们认为 TSPAN1 通过介导 EMT 和 PI3K/AKT 通路抑制乳腺癌的生长和迁移，可作为治疗乳腺癌的潜在靶点。
