Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
J Vet Med Sci. 2022 Jan 24;84(1):129-132. doi: 10.1292/jvms.21-0539. Epub 2021 Dec 2.
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
生殖道泰勒菌可引起马传染性子宫炎。本研究比较了七种针对生殖道泰勒菌的核酸扩增检测方法，旨在筛选出一种快速可靠的诊断方法。各检测方法的 95%检测限差异较大：实时 PCR 的检测限最低（0.77 fg/反应）；部分常规 PCR（cPCR）的检测限＞100 fg/反应。在感染实验样本中，实时 PCR 和半巢式 PCR 的阳性数量最高（42 个样本中有 33 个），但两种 cPCR 仅检测到 2 个和 7 个阳性结果。本研究结果表明，使用敏感的分子检测方法对于从临床样本中有效检测生殖道泰勒菌至关重要。
