使用荧光素衍生的氮氧自由基探针检测和结构分析 DNA 上生成的嘧啶衍生自由基。

Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe.

机构信息

Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582, Japan.

Metabolomics Laboratory, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka 812-8582, Japan.

出版信息

Chem Commun (Camb). 2021 Dec 21;58(1):56-59. doi: 10.1039/d1cc04998d.

DOI:10.1039/d1cc04998d

PMID:34897335

Abstract

The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.

摘要

DNA 的氧化损伤与衰老和各种疾病的发展有关。尽管核苷衍生的自由基在 DNA 氧化中起重要作用，但它们的分析方法有限。在此，我们提出了一种使用预荧光化的氮氧自由基探针结合液相色谱-荧光法和高分辨串联质谱法，对氧化损伤 DNA 表面的自由基进行荧光检测和结构分析的方法。