Fiehn N E, Oram V, Moe D
Acta Odontol Scand. 1986 Feb;44(1):1-9. doi: 10.3109/00016358609041291.
Thirty-eight young adults participated in the study. They were divided in a caries-inactive group, a low caries activity group and a moderate to high caries activity group. Total cultivable bacteria, Streptococcus salivarius, and S. mutans in plaque and saliva were quantitated on TSA, MS, and MSB plates, respectively. Sucrase activity was determined by measuring reducing sugars in plaque and saliva after incubation with sucrose. alpha-Amylase activity was determined by Pharmacia Phadebas Amylase test. The data were analyzed with the non-parametric Mann-Whitney U test. The only significant difference was observed for plaque alpha-amylase activity between the caries-inactive group and the moderate-high caries activity group (P less than 0.05). The lack of differences concerning the other variables is discussed mainly on the basis of the multifactorial character of dental caries and the possible insufficiency of the applied methods.
38名年轻人参与了这项研究。他们被分为龋齿非活动组、低龋齿活动组和中度至高度龋齿活动组。分别在TSA、MS和MSB平板上对菌斑和唾液中的可培养细菌总数、唾液链球菌和变形链球菌进行定量。蔗糖酶活性通过在与蔗糖孵育后测量菌斑和唾液中的还原糖来确定。α-淀粉酶活性通过Pharmacia Phadebas淀粉酶试验测定。数据采用非参数曼-惠特尼U检验进行分析。仅在龋齿非活动组和中度至高度龋齿活动组之间观察到菌斑α-淀粉酶活性存在显著差异（P<0.05）。关于其他变量缺乏差异主要基于龋齿的多因素特性以及所应用方法可能存在的不足进行了讨论。
