Department of Chemistry & Biochemistry, University of Windsor, Windsor, ON, Canada.
Methods Mol Biol. 2022;2402:151-161. doi: 10.1007/978-1-0716-1843-1_13.
The monomeric exchange kinetics of sub-micron particles provide insight into their stability and dynamism. Traditional techniques used to measure the intra- and inter-particle exchange often require monitoring the transfer of bulky and perturbing fluorescent labels. Time-resolved small angle neutron scattering (TR-SANS) overcomes these flaws by isotope labeling, allowing for the monomeric exchange rate determination of unperturbed, stress-free particles. Here, we describe TR-SANS in detail and novel applications of the technique.
亚微米颗粒的单体交换动力学为其稳定性和动态性提供了深入的了解。用于测量颗粒内和颗粒间交换的传统技术通常需要监测体积庞大且易干扰的荧光标记物的转移。通过同位素标记，时间分辨小角中子散射（TR-SANS）克服了这些缺陷，从而可以确定未受干扰、无应力的颗粒的单体交换率。在这里，我们详细描述了 TR-SANS 及其技术的新颖应用。
