CICS-UBI - Health Sciences Research Centre, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
CIEPQPF - Departamento de Engenharia Química, Universidade de Coimbra, Rua Sílvio Lima, 3030-790, Coimbra, Portugal.
Nanomedicine (Lond). 2021 Dec;16(29):2569-2586. doi: 10.2217/nnm-2021-0270. Epub 2021 Dec 2.
To develop a tumor-targeted chemo-photothermal nanomedicine through the functionalization of acridine orange (AO)-loaded gold-core mesoporous silica shell (AuMSS) nanorods with polyethylenimine (PEI) and hyaluronic acid (HA). Functionalization of the AuMSS nanorods was achieved through the chemical linkage of PEI followed by electrostatic adsorption of HA. HA functionalization improved AuMSS' cytocompatibility by decreasing blood hemolysis, and PEI-HA inclusion promoted a controlled and sustained AO release. assays revealed that HA functionalization increased the internalization of nanoparticles by human negroid cervix epithelioid carcinoma cancer (HeLa) cells, and the combinatorial treatment mediated by AuMSS/PEI/HA_AO nanorods presented an enhanced effect, with >95% of cellular death. AuMSS/PEI/HA_AO formulations can act as tumor-targeted chemo-photothermal nanomedicines for the combinatorial therapy of cervical cancer.
通过将载阿霉素（AO）的金核介孔硅壳（AuMSS）纳米棒进行聚乙二胺（PEI）和透明质酸（HA）功能化，开发一种肿瘤靶向化疗-光热纳米医学。通过 PEI 的化学连接，然后静电吸附 HA，实现了 AuMSS 纳米棒的功能化。HA 功能化通过降低血液溶血来提高 AuMSS 的细胞相容性，并且 PEI-HA 包封促进了 AO 的控制和持续释放。实验表明，HA 功能化增加了人黑人宫颈上皮样癌细胞（HeLa）对纳米颗粒的内化，并且由 AuMSS/PEI/HA_AO 纳米棒介导的组合治疗具有增强的效果，细胞死亡率超过 95%。AuMSS/PEI/HA_AO 制剂可用作肿瘤靶向化疗-光热纳米医学，用于宫颈癌的联合治疗。
