Department of Pharmaceutical Engineering, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China.
Department of Pharmaceutical Engineering, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Dec 1;81:261-270. doi: 10.1016/j.msec.2017.08.002. Epub 2017 Aug 2.
This work reports a multifunctional nanoplatform (GNRs@mSiO-HA-RGD) by conjugating targeting ligand hyaluronic acid (HA) and RGD with mesoporous silica-coated gold nanorods (GNRs@mSiO) for dual-targeted chemo-photothermal therapy. Doxorubicin hydrochloride (DOX) was used as the model drug to investigate the drug loading, in vitro drug release profiles and cell evaluation. The nanoplatform has demonstrated a good photothermal effect and excellent drug loading capacity of about 20.16%. It also had pH-enzyme sensitive and NIR-triggered drug release properties. Cellular uptake experiment results indicated that DOX-GNRs@mSiO-HA-RGD can be dual-targeted to ovarian cancer cells via CD44 and integrin receptor mediated endocytosis pathway. Cytotoxicity experiments demonstrated that combined therapy exhibited a better therapy effect compared to that of single chemotherapy or photothermal therapy. Our study demonstrates that DOX-GNRs@mSiO-HA-RGD may be a new promising dual-targeted delivery system for chemo-photothermal therapy.
这项工作报道了一种多功能纳米平台（GNRs@mSiO-HA-RGD），通过将靶向配体透明质酸（HA）和 RGD 与介孔硅包覆的金纳米棒（GNRs@mSiO）偶联，用于双重靶向化疗-光热治疗。盐酸阿霉素（DOX）被用作模型药物，以研究药物负载、体外药物释放曲线和细胞评价。该纳米平台表现出良好的光热效应和约 20.16%的优异载药能力。它还具有 pH 酶敏感和 NIR 触发的药物释放特性。细胞摄取实验结果表明，DOX-GNRs@mSiO-HA-RGD 可以通过 CD44 和整合素受体介导的内吞途径双重靶向卵巢癌细胞。细胞毒性实验表明，与单一化疗或光热治疗相比，联合治疗具有更好的治疗效果。我们的研究表明，DOX-GNRs@mSiO-HA-RGD 可能是一种新的有前途的化疗-光热治疗双重靶向递药系统。
