miR-21 通过靶向 PTEN 调节 BMSCs 的成骨和成脂分化。
miR-21 regulates osteogenic and adipogenic differentiation of BMSCs by targeting PTEN.
机构信息
Department of Orthopedic One Ward, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.
Department of Hemodialysis, Qiqihar Jian Hua Hospital, Qiqihar, China.
出版信息
J Musculoskelet Neuronal Interact. 2021 Dec 1;21(4):568-576.
OBJECTIVE
To explore the effects and mechanism of miR-21 on the osteogenic/adipogenic differentiation of mouse BMSCs.
METHODS
The bilateral ovaries of C57BL/6J mice (n=24) were removed to construct an osteoporosis model. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-21, osteogenic/adipogenic genes, and PTEN. ALP and ARS and ORO staining were used to detect the formation of calcium nodules and lipid droplets in BMSCs. Western blot was used to detect the expression of PTEN.
RESULTS
miR-21 was significantly down-regulated in osteoporotic mice. The expression of miR-21 was significantly up-regulated after the osteogenic induction of BMSCs, and the expression of miR-21 was significantly down-regulated after the adipogenic induction. Overexpression of miR-21 significantly promoted the osteogenic differentiation of BMSCs and inhibits the adipogenic differentiation of BMSCs.
CONCLUSION
MiR-21 can promote osteogenic differentiation of BMSCs and inhibit their adipogenic differentiation by negatively regulating PTEN.
目的
探讨 miR-21 对小鼠 BMSCs 成骨/成脂分化的影响及其作用机制。
方法
切除 C57BL/6J 小鼠双侧卵巢构建骨质疏松模型。实时定量聚合酶链反应（qRT-PCR）检测 miR-21、成骨/成脂基因和 PTEN 的表达。碱性磷酸酶（ALP）和酒石酸酸性磷酸酶（ARS）及油红 O（ORO）染色检测 BMSCs 中钙结节和脂滴的形成。Western blot 检测 PTEN 的表达。
结果
骨质疏松小鼠中 miR-21 表达显著下调。BMSCs 成骨诱导后 miR-21 表达显著上调，成脂诱导后 miR-21 表达显著下调。过表达 miR-21 可显著促进 BMSCs 的成骨分化，抑制 BMSCs 的成脂分化。
结论
miR-21 可通过负调控 PTEN 促进 BMSCs 的成骨分化，抑制其成脂分化。
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