National Research Center "Kurchatov Institute", Moscow, Russia.
Bull Exp Biol Med. 2021 Dec;172(2):228-235. doi: 10.1007/s10517-021-05368-0. Epub 2021 Dec 2.
We studied the effect of neural stem cells (NSC) and mesenchymal stem cells (MSC) from mouse adipose tissue on survival, clonogenic activity, and senescence of NSC after exposure to γ-radiation. It was found that survival and clonogenic activity of NSC irradiated in doses of 1 and 2 Gy was enhanced when irradiated cells were co-cultured with non-irradiated NSC and MSC in permeable Transwell inserts. The proportion of senescent NSC (cells with high β-galactosidase activity) increased with increasing irradiation dose. Co-culturing with non-irradiated NSC in 3 days after irradiation in a dose of 1 Gy led to a decrease in the proportion of senescent cells among irradiated NSC. Factors secreted by NSC and MSC can become the basis for the development of means for prevention and treatment of damage to brain cells resulting from radiation therapy of head and neck cancer.
我们研究了来自小鼠脂肪组织的神经干细胞 (NSC) 和间充质干细胞 (MSC) 对 NSC 在暴露于 γ 射线后存活、克隆形成活性和衰老的影响。结果发现，当用可渗透的 Transwell 插入物将照射细胞与未照射的 NSC 和 MSC 共培养时，1 和 2 Gy 剂量照射的 NSC 的存活率和克隆形成活性增强。随着照射剂量的增加，衰老的 NSC（β-半乳糖苷酶活性高的细胞）的比例增加。在 1 Gy 剂量照射后 3 天与未照射的 NSC 共培养会导致照射的 NSC 中衰老细胞的比例降低。NSC 和 MSC 分泌的因子可能成为开发预防和治疗头颈部癌症放射治疗引起的脑细胞损伤的方法的基础。
