Department of Wood and Paper, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Department of Food Chemistry and Technology, Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Int J Biol Macromol. 2022 Jan 15;195:49-58. doi: 10.1016/j.ijbiomac.2021.11.162. Epub 2021 Nov 29.
This study aimed to develop a plasticized starch (PS) based film loaded with chitosan nanoparticles (CNPs, 1, 2, 3, and 4%) as a reinforcing and antibacterial agent. We examined the morphology, biodegradability, mechanical, thermo-mechanical, and barrier properties of the PS/CNPs films. The antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria was investigated by colony forming unit (CFU) and disc diffusion methods. A dense structure was obtained for all PS/CNPs films and, thus, their complete biodegradation occurred in more days than neat PS. The increase in the CNPs percentage led to improved mechanical behaviour and barrier properties. PS-CNPs composite films revealed inhibition zones against both E. coli and S. aureus, with the 100% reduction in CFU against S. aureus. The current study exhibited that PS-CNPs films were more effective in inhibiting bacteria growth than neat PS film, confirming the composite films potential application as antimicrobial food packaging.
本研究旨在开发一种负载壳聚糖纳米粒子（CNPs，1%、2%、3%和 4%）的增塑淀粉（PS）基薄膜，作为增强和抗菌剂。我们研究了 PS/CNPs 薄膜的形态、可生物降解性、机械性能、热机械性能和阻隔性能。通过平板菌落计数法和抑菌圈法评估了 PS/CNPs 薄膜对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）的抗菌活性。所有 PS/CNPs 薄膜均呈现致密结构，因此其完全生物降解所需的时间均长于纯 PS 薄膜。随着 CNPs 百分比的增加，薄膜的机械性能和阻隔性能得到改善。PS-CNPs 复合薄膜对大肠杆菌和金黄色葡萄球菌均具有抑制作用，对金黄色葡萄球菌的 CFU 减少了 100%。本研究表明 PS-CNPs 薄膜在抑制细菌生长方面比纯 PS 薄膜更有效，证实了复合薄膜作为抗菌食品包装的潜在应用。
