Pedrosa-Silva Francisnei, Matteoli Filipe P, Passarelli-Araujo Hemanoel, Olivares Fabio L, Venancio Thiago M
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Brazil.
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Brazil; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Microbiol Res. 2021 Nov 18;255:126923. doi: 10.1016/j.micres.2021.126923.
The Stenotrophomonas maltophilia complex (Smc) is a cosmopolitan bacterial group that has been proposed an emergent multidrug-resistant pathogen. Taxonomic studies support the genomic heterogeneity of Smc, which comprises genogroups exhibiting a range of phenotypically distinct strains from different sources. Here, we report the genome sequencing and in-depth analysis of S. maltophilia UENF-4GII, isolated from vermicompost. This genome harbors a unique region encoding a penicillin-binding protein (pbpX) that was carried by a transposon, as well as horizontally-transferred genomic islands involved in anti-phage defense via DNA modification, and pili glycosylation. We also analyzed all available Smc genomes to investigate genes associated with resistance and virulence, niche occupation, and population structure. S. maltophilia UENF-4GII belongs to genogroup 3 (Sm3), which comprises three phylogenetic clusters (PC). Pan-GWAS analysis uncovered 471 environment-associated and 791 PC-associated genes, including antimicrobial resistance (e.g. blaL1 and blaR1) and virulence determinants (e.g. treS and katG) that provide insights on the resistance and virulence potential of Sm3 strains. Together, the results presented here provide the grounds for more detailed clinical and ecological investigations of S. maltophilia.
嗜麦芽窄食单胞菌复合体（Smc）是一个广泛分布的细菌类群，已被认为是一种新出现的多重耐药病原体。分类学研究支持Smc的基因组异质性，它由基因组群组成，这些基因组群包含来自不同来源的一系列表型不同的菌株。在此，我们报告了从蚯蚓堆肥中分离出的嗜麦芽窄食单胞菌UENF-4GII的全基因组测序及深入分析。该基因组含有一个独特区域，编码一个由转座子携带的青霉素结合蛋白（pbpX），以及通过DNA修饰参与抗噬菌体防御和菌毛糖基化的水平转移基因组岛。我们还分析了所有可用的Smc基因组，以研究与抗性、毒力、生态位占据和种群结构相关的基因。嗜麦芽窄食单胞菌UENF-4GII属于基因组群3（Sm3），其包含三个系统发育簇（PC）。全基因组关联分析（Pan-GWAS）发现了471个与环境相关的基因和791个与PC相关的基因，包括抗微生物抗性（如blaL1和blaR1）和毒力决定因素（如treS和katG），这些基因有助于深入了解Sm3菌株的抗性和毒力潜力。总之，本文给出的结果为对嗜麦芽窄食单胞菌进行更详细的临床和生态学研究提供了依据。
