Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China.
School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing, 211166, China.
BMC Med Genomics. 2021 Dec 2;14(Suppl 2):276. doi: 10.1186/s12920-021-01132-5.
Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma.
We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function.
15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA-miRNA network suggested that 4 special circRNAs could be used as potential biomarkers.
Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA-miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment.
卵巢浆液性囊腺癌是妇科最严重的恶性肿瘤之一。环状 RNA（circRNA）是一种具有共价闭合连续环结构的非编码 RNA。circRNA 的异常表达可能与肿瘤发生有关，因为其复杂的生物学机制，例如作为 microRNA（miRNA）海绵发挥作用。然而，卵巢浆液性囊腺癌中的 circRNA 表达谱及其与其他 RNA 的关联尚未得到表征。本研究的主要目的是揭示卵巢浆液性囊腺癌中的 circRNA 表达谱。
我们从 3 名卵巢浆液性囊腺癌患者的 6 个标本中收集了组织。经过 RNA 测序，我们分析了 circRNA 与相关 mRNAs 和 miRNAs 的表达，以描述潜在的功能。
在 6 个标本中鉴定出 15092 个独特的 circRNA。这些 circRNA 中有约 46%未记录在公共数据库中。我们随后报告了 353 个差异表达的 circRNA，其中包含癌基因和肿瘤抑制基因。此外，与相关 mRNAs 的联合分析表明 circRNA 与其同源 mRNAs 之间存在一致的变化。总的来说，circRNA-miRNA 网络的构建表明 4 个特殊的 circRNA 可以用作潜在的生物标志物。
我们的研究揭示了卵巢浆液性囊腺癌患者组织中的 circRNA 表达谱。差异表达的 circRNA 被认为与卵巢浆液性囊腺癌在富集分析中有关，与相关 mRNAs 和 miRNAs 的共表达分析说明了潜在的调控网络。我们还构建了一个复杂的 circRNA-miRNA 相互作用网络，然后证明了某些 circRNA 的潜在功能，以辅助未来的诊断和治疗。
