Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, 106, Taiwan.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 106, Taiwan; Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, 106, Taiwan.
Anal Chim Acta. 2022 Jan 15;1190:339244. doi: 10.1016/j.aca.2021.339244. Epub 2021 Nov 3.
Salmonella contamination is a major concern in food and public health safety, and carrying out episodic monitoring of Salmonella contamination in food and water bodies is essential for safeguarding public health and the economy. Therefore, there is an urgent need to develop an easy-to-operate Salmonella-targeting point-of-care detection platform. To this end, we designed two activity-based latent ratiometric electrochemical molecular substrates, denoted as Sal-CAF and Sal-NBAF, specifically for achieving easy, rapid, and selective profiling of Salmonella esterase (a Salmonella biomarker) under physiological conditions. The octyl esters of the substrates were cleaved by the esterase and triggered the trimethyl lock to eject the electron-rich aminoferrocene derivatives (CAF and NBAF), and the corresponding electrochemical signals were tracked at the negative region (-0.08 V vs Ag/AgCl) of the voltammetric spectrum. The Sal-CAF substrate was used to determine the concentration of Salmonella in a wide dynamic range (1.03 × 10-1.1 × 10 CFU mL) with a low detection limit of 39.27 × 10 CFU mL. The developed probes were tested against various bacteria but were only activated by live Salmonella. Furthermore, the Sal-CAF probe was used directly in quantifying spiked live Salmonella spiked in milk samples and also used to effectively monitor and quantify Salmonella production in real-time. These achievements indicated the Sal-CAF probe to be a promising platform for point-of-care Salmonella analysis.
沙门氏菌污染是食品安全和公共卫生安全的主要关注点，对食品和水体中的沙门氏菌污染进行间歇性监测对于保障公众健康和经济至关重要。因此，迫切需要开发一种易于操作的针对沙门氏菌的即时检测平台。为此，我们设计了两种基于活性的潜伏比率电化学分子底物，分别表示为 Sal-CAF 和 Sal-NBAF，专门用于在生理条件下轻松、快速和选择性地分析沙门氏菌酯酶（一种沙门氏菌生物标志物）。底物的辛酯被酯酶切割，触发三甲基锁释放富电子氨基二茂铁衍生物（CAF 和 NBAF），并在伏安光谱的负区域（-0.08 V 对 Ag/AgCl）跟踪相应的电化学信号。Sal-CAF 底物用于在宽动态范围内（1.03 × 10-1.1 × 10 CFU mL）测定沙门氏菌的浓度，检测限低至 39.27 × 10 CFU mL。开发的探针针对各种细菌进行了测试，但仅被活的沙门氏菌激活。此外，Sal-CAF 探针直接用于定量牛奶样品中添加的活沙门氏菌，也用于实时有效监测和定量沙门氏菌的产生。这些结果表明 Sal-CAF 探针是一种用于即时分析沙门氏菌的有前途的平台。
