Nanotechnology and Integrated Bio-Engineering Centre (NIBEC), Ulster University, Jordanstown Campus -, Newtownabbey BT37 0QB, UK; School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
Mater Sci Eng C Mater Biol Appl. 2021 Dec;131:112523. doi: 10.1016/j.msec.2021.112523. Epub 2021 Oct 26.
Current surgical strategies for the treatment of pelvic floor dysfunctions involve the placement of a polypropylene mesh into the pelvic cavity. However, polypropylene meshes have proven to have inadequate mechanical properties and have been associated to the arising of severe complications, such as infections. Furthermore, currently employed manufacturing strategies are unable to produce compliant and customisable devices. In this work, polycaprolactone has been used to produce resorbable levofloxacin-loaded meshes in two different designs (90° and 45°) via melt-extrusion 3D printing. Drug-loaded meshes were produced using a levofloxacin concentration of 0.5% w/w. Drug loaded meshes were successfully produced with highly reproducible mechanical and physico-chemical properties. Tensile test results showed that drug-loaded 45° meshes possessed a mechanical behaviour close to that of the vaginal tissue (E ≃ 8.32 ± 1.85 MPa), even after 4 weeks of accelerated degradation. Meshes released 80% of the loaded levofloxacin in the first 3 days and were capable of producing an inhibitory effect against S. Aureus and E. coli bacterial strains with an inhibition zone equal to 12.8 ± 0.45 mm and 15.8 ± 0.45 mm respectively. Thus, the strategy adopted in this work holds great promise for the manufacturing of custom-made surgical meshes with antibacterial properties.
目前治疗盆底功能障碍的手术策略包括将聚丙烯网片置于盆腔内。然而，聚丙烯网片已被证明其机械性能不足，并与严重并发症的出现有关，如感染。此外，目前采用的制造策略无法生产出具有顺应性和可定制的装置。在这项工作中，聚己内酯被用于通过熔融挤出 3D 打印生产两种不同设计（90°和 45°）的可吸收左氧氟沙星负载网片。使用 0.5%w/w 的左氧氟沙星浓度制备载药网片。成功地制备了具有高度可重现的机械和物理化学性能的载药网片。拉伸试验结果表明，负载药物的 45°网片具有接近阴道组织的机械性能（E≃8.32±1.85MPa），即使在加速降解 4 周后也是如此。网片在头 3 天内释放了 80%的负载左氧氟沙星，并能够对金黄色葡萄球菌和大肠杆菌菌株产生抑制作用，抑菌圈分别为 12.8±0.45mm 和 15.8±0.45mm。因此，这项工作中采用的策略为制造具有抗菌性能的定制手术网片提供了很大的希望。
