Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, 12 Jiangan Road, Guilin, 541004, PR China.
Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, 12 Jiangan Road, Guilin, 541004, PR China.
Talanta. 2022 Feb 1;238(Pt 1):122999. doi: 10.1016/j.talanta.2021.122999. Epub 2021 Oct 29.
This work initially reports a new nanosening method for simple, sensitive, specific, visual detection of mercury (II) (Hg) and glutathione (GSH) using the Tyndall Effect (TE) of the same colloidal gold nanoparticle (GNP) probes for efficient colorimetric signaling amplification. For the TE-inspired assay (TEA) method, arginine (Arg) molecules are pre-modified on the GNPs' surfaces (Arg-GNPs). Upon the Hg introduction, it can be specifically coordinated with the terminal -NH and -COOH groups of the Arg molecules to make the Arg-GNPs aggregate, producing a significantly-enhanced TE signal in the reaction solution after its irradiation by a 635-nm red laser pointer pen. On the other hand, the introduction of the GSH results in the production of the original Arg-GNPs' weak TE response, as it is able to bind such metal ion via mercury-thiol reactions to inhibit the above aggregation. Under the optimal conditions, the utility of the new TEA method is well demonstrated to quantitatively detect the Hg and GSH with the aid of a smartphone as a portable TE reader during the linear concentration ranges of 50-3000 and 10-3000 nM, respectively. The detection limits for the Hg and GSH are estimated to be as low as ∼3.5 and ∼0.3 nM, respectively. The recovery results obtained from the detection of Hg in the complex tap and pond water samples and the assay of GSH in real human serum and urine samples are also satisfactory.
本工作首次报道了一种新的纳米传感方法，用于简单、灵敏、特异、可视化检测汞（II）（Hg）和谷胱甘肽（GSH），使用胶体金纳米粒子（GNP）探针的丁达尔效应（TE）进行高效比色信号放大。对于基于 TE 的分析（TEA）方法，精氨酸（Arg）分子预先修饰在 GNP 表面（Arg-GNP）上。引入 Hg 后，它可以与 Arg 分子的末端 -NH 和 -COOH 基团特异性配位，使 Arg-GNP 聚集，在 635nm 红色激光笔照射后，反应溶液中的 TE 信号显著增强。另一方面，GSH 的引入导致原始 Arg-GNP 的 TE 响应减弱，因为它能够通过汞-硫醇反应与金属离子结合，从而抑制上述聚集。在最佳条件下，借助智能手机作为便携式 TE 阅读器，新的 TEA 方法可用于定量检测 Hg 和 GSH，线性浓度范围分别为 50-3000 和 10-3000 nM。Hg 和 GSH 的检测限分别估计低至约 3.5 和约 0.3 nM。从复杂的自来水和池塘水样中检测 Hg 和从实际人血清和尿样中测定 GSH 的回收结果也令人满意。
