Wu Pian, Huang Ruixue, Chen Cuimei, Yi Jiecan, Kai Tianhan, Zhan Yujuan, Wei Xiaoqian, Wang Danqi, Zhang Jingwen, Ding Ping
Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China.
Mikrochim Acta. 2022 Oct 7;189(11):408. doi: 10.1007/s00604-022-05494-0.
A novel aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide (CMC/GO@Apt-Au NP) probe was for the first time developed for the determination of Salmonella typhimurium (S. typhimurium). Owing to the conformational change of the aptamers in the presence of S. typhimurium, the Au NPs, which were pre-adsorbed on the aptamers through van der Waals forces, were released into the solution phase and induced the color change of the solution. As a result, S. typhimurium ranging from 10 to 10 CFU/mL was successfully identified using the designed assay with a limit of detection (LOD) of 10 CFU/mL. This low detection level allowed the sensitive recognition of S. typhimurium in milk samples within 40 min without sample pretreatment, a conclusion that agreed well with the traditional plate counting method. The developed method not only provides a rapid way for the determination of S. typhimurium with simplicity and sensitivity but also shows potential universality in the quantification of other pathogenic microorganisms.
首次开发了一种新型适体-金纳米颗粒共轭羧甲基壳聚糖功能化氧化石墨烯（CMC/GO@Apt-Au NP）探针，用于检测鼠伤寒沙门氏菌（S. typhimurium）。由于在鼠伤寒沙门氏菌存在下适体的构象变化，通过范德华力预先吸附在适体上的金纳米颗粒被释放到溶液相中，导致溶液颜色变化。结果，使用设计的检测方法成功鉴定出浓度范围为10至10 CFU/mL的鼠伤寒沙门氏菌，检测限（LOD）为10 CFU/mL。这种低检测水平使得无需样品预处理即可在40分钟内灵敏识别牛奶样品中的鼠伤寒沙门氏菌，这一结论与传统平板计数法吻合良好。所开发的方法不仅为鼠伤寒沙门氏菌的测定提供了一种简单、灵敏的快速方法，而且在其他致病微生物的定量分析中也显示出潜在的通用性。
