长链非编码 RNA CASC9-1 通过靶向 miR-383-5p 上调 MAPKAP1 促进宫颈鳞状细胞癌中的细胞恶性行为。

LncRNA CASC9-1 Facilitates Cell Malignant Behaviors in Cervical Squamous Cell Carcinoma by Targeting miR-383-5p to Up-regulate MAPKAP1.

机构信息

Department of Obsterics, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

出版信息

Arch Med Res. 2022 Feb;53(2):138-146. doi: 10.1016/j.arcmed.2021.10.008. Epub 2021 Nov 30.

DOI:10.1016/j.arcmed.2021.10.008

PMID:34857398

Abstract

BACKGROUND

Cervical squamous cell carcinoma (Cervical SCC) is a malignant gynecological tumor, which greatly endangers the health of global women. Cancer susceptibility candidate 9 (CASC9) has been identified as an oncogene in multiple cancers. However, the role of CASC9-1 (one transcript of CASC9) in cervical SCC remains covered.

METHODS

We analyzed gene expression in cervical SCC cells via RT-qPCR and western blot assays. The impact of CASC9-1 on cervical SCC cell and tumor growth was assessed via in vitro functional assays and in vivo assays. Moreover, the regulatory mechanism of CASC9-1 was explored by mechanism assays.

RESULTS

CASC9-1 was up-regulated in cervical SCC cells. CASC9-1 knockdown repressed cervical SCC cell proliferation, migration and invasion while elevating apoptosis. Via in-vivo experiments, CASC9-1 down-regulation was proved to restrict cervical SCC tumor growth. In terms of mechanism, CASC9-1 directly targeted miR-383-5p, and MAPKAP1 was the target gene of miR-383-5p in cervical SCC cells. CASC9-1 could exacerbate malignant behaviors of cervical SCC cells via binding to miR-383-5p and regulating MAPKAP1.

CONCLUSIONS

CASC9-1 exerted influences on various biological behaviors of cervical SCC cells via targeting miR-383-5p to up-regulate MAPKAP1. All these results mirrored that CASC9-1 might be a potential target for cervical SCC treatment.

摘要

背景

宫颈鳞状细胞癌（Cervical SCC）是一种恶性妇科肿瘤，严重威胁着全球女性的健康。癌症易感性候选基因 9（CASC9）已被确定为多种癌症的癌基因。然而，CASC9-1（CASC9 的一个转录本）在宫颈鳞癌中的作用仍不清楚。

方法

我们通过 RT-qPCR 和 Western blot 分析了宫颈鳞癌细胞中的基因表达。通过体外功能测定和体内测定评估了 CASC9-1 对宫颈鳞癌细胞和肿瘤生长的影响。此外，通过机制测定探讨了 CASC9-1 的调控机制。

结果

CASC9-1 在宫颈鳞癌细胞中上调。CASC9-1 敲低抑制宫颈鳞癌细胞的增殖、迁移和侵袭，同时促进凋亡。通过体内实验证明，CASC9-1 下调可抑制宫颈鳞癌肿瘤生长。在机制方面，CASC9-1 可直接靶向 miR-383-5p，而 MAPKAP1 是 miR-383-5p 在宫颈鳞癌细胞中的靶基因。CASC9-1 可通过与 miR-383-5p 结合并调节 MAPKAP1 来加重宫颈鳞癌细胞的恶性行为。